| Enhanced seed viability and lipid compositional changes during natural ageing by suppressing phospholipase Dα in soybean seed. | |
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MedLine Citation:
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PMID: 21895945 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Changes in phospholipid composition and consequent loss of membrane integrity are correlated with loss of seed viability. Furthermore, phospholipid compositional changes affect the composition of the triacylglycerols (TAG), i.e. the storage lipids. Phospholipase D (PLD) catalyses the hydrolysis of phospholipids to phosphatidic acid, and PLDα is an abundant PLD isoform. Although wild-type (WT) seeds stored for 33 months were non-viable, 30%-50% of PLDα-knockdown (PLD-KD) soybean seeds stored for 33 months germinated. WT and PLD-KD seeds increased in lysophospholipid levels and in TAG fatty acid unsaturation during ageing, but the levels of lysophospholipids increased more in WT than in PLD-KD seeds. The loss of viability of WT seeds was correlated with alterations in ultrastructure, including detachment of the plasma membrane from the cell wall complex and disorganization of oil bodies. The data demonstrate that, during natural ageing, PLDα affects the soybean phospholipid profile and the TAG profile. Suppression of PLD activity in soybean seed has potential for improving seed quality during long-term storage. |
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Authors:
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Junghoon Lee; Ruth Welti; Mary Roth; William T Schapaugh; Jiarui Li; Harold N Trick |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-5 |
Journal Detail:
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Title: Plant biotechnology journal Volume: - ISSN: 1467-7652 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101201889 Medline TA: Plant Biotechnol J Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 The Authors. Plant Biotechnology Journal © 2011 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd. |
Affiliation:
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Department of Plant Pathology, Kansas State University, Manhattan, KS, USA Kansas Lipidomics Research Center, Division of Biology, Kansas State University, Manhattan, KS, USA Department of Agronomy, Kansas State University, Manhattan, KS, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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