Document Detail


Enhanced seed viability and lipid compositional changes during natural ageing by suppressing phospholipase Dα in soybean seed.
MedLine Citation:
PMID:  21895945     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Changes in phospholipid composition and consequent loss of membrane integrity are correlated with loss of seed viability. Furthermore, phospholipid compositional changes affect the composition of the triacylglycerols (TAG), i.e. the storage lipids. Phospholipase D (PLD) catalyses the hydrolysis of phospholipids to phosphatidic acid, and PLDα is an abundant PLD isoform. Although wild-type (WT) seeds stored for 33 months were non-viable, 30%-50% of PLDα-knockdown (PLD-KD) soybean seeds stored for 33 months germinated. WT and PLD-KD seeds increased in lysophospholipid levels and in TAG fatty acid unsaturation during ageing, but the levels of lysophospholipids increased more in WT than in PLD-KD seeds. The loss of viability of WT seeds was correlated with alterations in ultrastructure, including detachment of the plasma membrane from the cell wall complex and disorganization of oil bodies. The data demonstrate that, during natural ageing, PLDα affects the soybean phospholipid profile and the TAG profile. Suppression of PLD activity in soybean seed has potential for improving seed quality during long-term storage.
Authors:
Junghoon Lee; Ruth Welti; Mary Roth; William T Schapaugh; Jiarui Li; Harold N Trick
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-09-05
Journal Detail:
Title:  Plant biotechnology journal     Volume:  10     ISSN:  1467-7652     ISO Abbreviation:  Plant Biotechnol. J.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-03     Completed Date:  2012-07-06     Revised Date:  2013-08-16    
Medline Journal Info:
Nlm Unique ID:  101201889     Medline TA:  Plant Biotechnol J     Country:  England    
Other Details:
Languages:  eng     Pagination:  164-73     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors. Plant Biotechnology Journal © 2011 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.
Affiliation:
Department of Plant Pathology, Kansas State University, Manhattan, KS, USA.
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Gene Knockdown Techniques
Lipid Metabolism
Phospholipase D / antagonists & inhibitors*,  genetics,  metabolism*
Phospholipids / metabolism*
Plants, Genetically Modified
Seeds / enzymology,  genetics,  metabolism*
Soybeans / enzymology,  genetics,  metabolism*
Time Factors
Grant Support
ID/Acronym/Agency:
P20 GM103418/GM/NIGMS NIH HHS; P20 RR016475/RR/NCRR NIH HHS; P20RR16475/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Phospholipids; EC 3.1.4.4/Phospholipase D
Comments/Corrections

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