Document Detail

Enhanced seed viability and lipid compositional changes during natural ageing by suppressing phospholipase Dα in soybean seed.
MedLine Citation:
PMID:  21895945     Owner:  NLM     Status:  MEDLINE    
Changes in phospholipid composition and consequent loss of membrane integrity are correlated with loss of seed viability. Furthermore, phospholipid compositional changes affect the composition of the triacylglycerols (TAG), i.e. the storage lipids. Phospholipase D (PLD) catalyses the hydrolysis of phospholipids to phosphatidic acid, and PLDα is an abundant PLD isoform. Although wild-type (WT) seeds stored for 33 months were non-viable, 30%-50% of PLDα-knockdown (PLD-KD) soybean seeds stored for 33 months germinated. WT and PLD-KD seeds increased in lysophospholipid levels and in TAG fatty acid unsaturation during ageing, but the levels of lysophospholipids increased more in WT than in PLD-KD seeds. The loss of viability of WT seeds was correlated with alterations in ultrastructure, including detachment of the plasma membrane from the cell wall complex and disorganization of oil bodies. The data demonstrate that, during natural ageing, PLDα affects the soybean phospholipid profile and the TAG profile. Suppression of PLD activity in soybean seed has potential for improving seed quality during long-term storage.
Junghoon Lee; Ruth Welti; Mary Roth; William T Schapaugh; Jiarui Li; Harold N Trick
Related Documents :
11731335 - Studies on the transport of acetyl groups from peroxisomes to mitochondria in isolated ...
9140835 - Acute and chronic effects of adriamycin on fatty acid oxidation in isolated cardiac myo...
12956315 - Mitochondrial and peroxisomal fatty acid oxidation capacities increase in the skeletal ...
7556205 - Phytanic acid alpha-oxidation in rat liver peroxisomes. production of alpha-hydroxyphyt...
11334565 - Antimycobacterial activity of substituted isosteres of pyridine- and pyrazinecarboxylic...
667735 - Utilization of oleuropein by microorganisms associated with olive fermentations.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-09-05
Journal Detail:
Title:  Plant biotechnology journal     Volume:  10     ISSN:  1467-7652     ISO Abbreviation:  Plant Biotechnol. J.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-03     Completed Date:  2012-07-06     Revised Date:  2013-08-16    
Medline Journal Info:
Nlm Unique ID:  101201889     Medline TA:  Plant Biotechnol J     Country:  England    
Other Details:
Languages:  eng     Pagination:  164-73     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors. Plant Biotechnology Journal © 2011 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.
Department of Plant Pathology, Kansas State University, Manhattan, KS, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Age Factors
Gene Knockdown Techniques
Lipid Metabolism
Phospholipase D / antagonists & inhibitors*,  genetics,  metabolism*
Phospholipids / metabolism*
Plants, Genetically Modified
Seeds / enzymology,  genetics,  metabolism*
Soybeans / enzymology,  genetics,  metabolism*
Time Factors
Grant Support
Reg. No./Substance:
0/Phospholipids; EC D

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Resistance to Wheat streak mosaic virus generated by expression of an artificial polycistronic micro...
Next Document:  Field performance of transgenic sugarcane expressing isomaltulose synthase.