Document Detail

Enhanced repair of DNA interstrand crosslinks in S phase.
MedLine Citation:
PMID:  16494874     Owner:  NLM     Status:  MEDLINE    
Hela cells synchronized in G1 and S phases of the cell cycle were transfected with pEGFP crosslinked with trioxsalen. Twelve hours later the number of fluorescent cells was determined by fluorescent microscopy. Cells in S phase have repaired 0.2-0.3 ICL/kb over the 12h period, while cells in G1 phase repaired interstrand crosslinks much more poorly. The crosslinked plasmids were efficiently recruited to the nuclear matrix both in G1 phase and S-phase, which showed that the poor repair of G1 cells was a result of a lack of DNA replication rather than of a lack of matrix attachment.
Veronika Mladenova; George Russev
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-17
Journal Detail:
Title:  FEBS letters     Volume:  580     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-28     Completed Date:  2006-04-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1631-4     Citation Subset:  IM    
Institute of Molecular Biology, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Block 21, 1113 Sofia, Bulgaria.
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MeSH Terms
Cross-Linking Reagents / chemistry
DNA Adducts / analysis
DNA Repair*
DNA Replication*
G1 Phase / genetics
Green Fluorescent Proteins / analysis,  genetics
Hela Cells
Plasmids / chemistry,  genetics
S Phase / genetics*
Trioxsalen / chemistry
Reg. No./Substance:
0/Cross-Linking Reagents; 0/DNA Adducts; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins; 3902-71-4/Trioxsalen

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