| Enhanced repair of DNA interstrand crosslinks in S phase. | |
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MedLine Citation:
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PMID: 16494874 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hela cells synchronized in G1 and S phases of the cell cycle were transfected with pEGFP crosslinked with trioxsalen. Twelve hours later the number of fluorescent cells was determined by fluorescent microscopy. Cells in S phase have repaired 0.2-0.3 ICL/kb over the 12h period, while cells in G1 phase repaired interstrand crosslinks much more poorly. The crosslinked plasmids were efficiently recruited to the nuclear matrix both in G1 phase and S-phase, which showed that the poor repair of G1 cells was a result of a lack of DNA replication rather than of a lack of matrix attachment. |
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Authors:
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Veronika Mladenova; George Russev |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-02-17 |
Journal Detail:
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Title: FEBS letters Volume: 580 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-02-28 Completed Date: 2006-04-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1631-4 Citation Subset: IM |
Affiliation:
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Institute of Molecular Biology, Bulgarian Academy of Sciences, Acad. G. Bonchev Street, Block 21, 1113 Sofia, Bulgaria. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cross-Linking Reagents
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chemistry DNA Adducts / analysis DNA Repair* DNA Replication* G1 Phase / genetics Green Fluorescent Proteins / analysis, genetics Hela Cells Humans Plasmids / chemistry, genetics S Phase / genetics* Trioxsalen / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Cross-Linking Reagents; 0/DNA Adducts; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins; 3902-71-4/Trioxsalen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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