Document Detail

Enhanced renal tubular ouabain-sensitive ATPase in streptozotocin diabetes mellitus.
MedLine Citation:
PMID:  3014897     Owner:  NLM     Status:  MEDLINE    
The effect of streptozotocin-induced diabetes mellitus on rat renal ouabain-sensitive ATPase in six distinct nephron segments was studied. Twenty-four hours after administration of streptozotocin, blood glucose increased threefold (P less than 0.001), and glucosuria was evident. Aldosterone levels increased almost twofold (P less than 0.001). Ouabain-sensitive ATPase increased in the proximal segments PC (proximal convoluted tubule) and PS (proximal straight tubule) by 43 and 62%, respectively, (P less than 0.001) and CD (cortical collecting duct) ouabain-sensitive ATPase increased 77% (P less than 0.001). Ouabain-sensitive ATPase in the cortical (CTAL) and medullary (MTAL) thick ascending limbs of Henle's loop and in the DC (distal convoluted tubule) remained unchanged after 24 h of streptozotocin administration. Eight days after streptozotocin administration, when glomerular filtration rate (GFR) was already markedly elevated, ouabain-sensitive ATPase remained increased in the PC, PS, and CD but was significantly less compared with the activity after 24 h (P less than 0.05), whereas in the CTAL and MTAL a marked increase in ouabain-sensitive ATPase occurred by 54% in the CTAL and 65% in the MTAL (P less than 0.001). Aldosterone levels remained elevated compared with control but less than after 24 h. Pretreatment with deoxycorticosterone acetate abolished the increase in ouabain-sensitive ATPase in the CD. These findings show that streptozotocin-induced diabetes mellitus in the rat is associated with a substantial increase in ouabain-sensitive ATPase activity along most of the nephron. This increase in enzyme activity may represent a mechanism of physiological adaptation of the nephron to maintain electrolyte homeostasis in diabetes in face of the increased GFR and osmotic diuresis.
H Wald; P Scherzer; M M Popovtzer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of physiology     Volume:  251     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1986 Jul 
Date Detail:
Created Date:  1986-08-21     Completed Date:  1986-08-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  F164-70     Citation Subset:  IM    
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MeSH Terms
Aldosterone / blood
Blood Glucose / analysis
Desoxycorticosterone / pharmacology
Diabetes Mellitus, Experimental / enzymology*
Glomerular Filtration Rate
Kidney Cortex / enzymology
Kidney Medulla / enzymology
Kidney Tubules / enzymology*
Loop of Henle / enzymology
Nephrons / enzymology
Ouabain / pharmacology*
Sodium-Potassium-Exchanging ATPase / metabolism*
Reg. No./Substance:
0/Blood Glucose; 52-39-1/Aldosterone; 630-60-4/Ouabain; 64-85-7/Desoxycorticosterone; EC ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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