Document Detail


Enhanced release of thromboxane A(2) after exposure of human airway epithelial cells to meconium.
MedLine Citation:
PMID:  11802247     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Meconium aspiration syndrome (MAS) is a cause of significant morbidity and mortality in the perinatal period. Despite the clinical relevance of MAS, its pathogenesis is poorly understood. Epithelial cell-derived prostanoids are involved in the regulation of several cellular functions within the lung, including the control of tone and reactivity of airway and vascular smooth muscle. In this study, we evaluated whether exposure to meconium affects the metabolic function of human airway epithelial cells. Monolayers of A549 cells, a transformed human epithelial cell line, were incubated with various concentrations of meconium. Control cells were incubated with serum-free medium in a similar manner. The supernatant fluid was removed at various time points and assayed for thromboxane A(2) (TXA(2)) production. The latter was accomplished by measuring its immediate and stable metabolite thromboxane B(2), using an enzyme-linked immunosorbent assay (ELISA). In selected experiments, the modulatory effects of indomethacin (10(-6) M), dexamethasone (10(-6) M), and L-nitroarginine methyl ester (L-NAME, 10(-6) M) on TXA(2) production were evaluated. Results were expressed in terms of pg/mg protein (mean +/- SE). We found that exposure to meconium produced a significant release of TXA(2) from A549 cells. Indomethacin, dexamethasone, and in part, L-NAME inhibited meconium-induced release of TXA(2). Our findings demonstrate that meconium enhances the production of thromboxanes from A549 cells, suggesting that airway epithelial cells and their metabolic products may play an important role in the pathogenesis of MAS.
Authors:
Amir M Khan; Kevin P Lally; Gary L Larsen; Giuseppe N Colasurdo
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Pediatric pulmonology     Volume:  33     ISSN:  8755-6863     ISO Abbreviation:  Pediatr. Pulmonol.     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-01-21     Completed Date:  2002-03-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8510590     Medline TA:  Pediatr Pulmonol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  111-6     Citation Subset:  IM    
Copyright Information:
Copyright 2002 Wiley-Liss, Inc.
Affiliation:
Department of Pediatrics, University of Texas-Houston Medical School, Houston, Texas 77030, USA.
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MeSH Terms
Descriptor/Qualifier:
Dexamethasone / pharmacology
Epithelial Cells / metabolism*
Humans
Indomethacin / pharmacology
Infant, Newborn
Lung Neoplasms / metabolism*
Meconium*
NG-Nitroarginine Methyl Ester / pharmacology
Thromboxane A2 / antagonists & inhibitors,  biosynthesis*
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
HL-03196/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
50-02-2/Dexamethasone; 50903-99-6/NG-Nitroarginine Methyl Ester; 53-86-1/Indomethacin; 57576-52-0/Thromboxane A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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