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Enhanced protection against pulmonary mycobacterial challenge by chitosan-formulated polyepitope gene vaccine was associated with elevated pulmonary SIgA and IFN-γ(+) T cell response.
MedLine Citation:
PMID:  23323767     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Induction of local (pulmonary) immunity plays a critical role in preventing dissemination of Mycobacterium tuberculosis (M. tb) at the early infection stage. On the basis of our previously constructed multi-epitope gene vaccine, pHSP65pep, which effectively induced splenic IFN-γ(+) Th1 response, chitosan, one natural cationic polysaccharide was employed as mucosal gene carrier to complex with pHSP65pep to induce the specific mucosal immunity. Chitosan-pHSP65pep was intranasally administered to BALB/c mice with 4 doses of 50μg DNA followed by mycobacterial challenge 4 weeks after the final immunization. It was found that chitosan formulation significantly induced the production of SIgA (P < 0.05) in the lung lavage and enhanced pulmonary CD4(+) as well as CD8(+) IFN-γ(+) T cell response (P < 0.001) compared with naked gene vaccine. In consistency, improved protection against Mycobacterium bovis BCG challenge was achieved by chitosan-DNA formulation both in vaccine alone or BCG-prime-vaccine boost immunization scenario. Our study indicated that mucosal delivery of gene vaccine in chitosan formulation remarkably enhanced specific SIgA level and mucosal IFN-γ(+) T cell response which were positively correlated with immunological protection.
Authors:
Wenqing Ai; Yan Yue; Sidong Xiong; Wei Xu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-17
Journal Detail:
Title:  Microbiology and immunology     Volume:  -     ISSN:  1348-0421     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Societies and Blackwell Publishing Asia Pty Ltd.
Affiliation:
Institute for Immunobiology, Shanghai Medical College, Fudan University, Shanghai, P. R. China.
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