Document Detail


Enhanced proliferation, attachment and osteopontin expression by porcine periodontal cells exposed to Emdogain.
MedLine Citation:
PMID:  16342405     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Emdogain (EMD) is an enamel matrix derivative extracted from developing porcine teeth with demonstrated periodontal regenerative potential. EMD has been shown to influence a number of properties of periodontal ligament cells including proliferation, cell attachment and matrix synthesis. To date, the effect of EMD on the epithelial cell rests of Malassez (ERM) is unknown. In this study, periodontal ligament fibroblasts, ERM, alveolar bone cells and gingival fibroblasts were obtained from porcine periodontal ligament, alveolar bone and gingiva. This study investigated, in vitro, the effect of EMD at three concentrations on proliferation, cell attachment and expression of mRNA for two mineralised tissue-related proteins (osteopontin and bone sialoprotein). As for other periodontal cells, the ERM proliferative response was enhanced by EMD. Attachment assays revealed a highly significant increase for ERM and gingival fibroblasts after EMD treatment at all concentrations. This study has also shown that EMD stimulated expression of osteopontin mRNA by ERM and alveolar bone cells. The results from this study provide evidence that EMD enhanced cellular events related with proliferation, attachment and osteopontin mRNA expression by porcine periodontal cells, in a manner consistent with its role in periodontal regenerative therapy.
Authors:
J C Rincon; Y Xiao; W G Young; P M Bartold
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of oral biology     Volume:  50     ISSN:  0003-9969     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-07     Completed Date:  2006-03-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1047-54     Citation Subset:  D; IM    
Affiliation:
Department of Dentistry, University of Queensland, Brisbane, Qld, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion
Cell Proliferation
Cells, Cultured
DNA / biosynthesis
Dental Enamel Proteins / pharmacology*
Fibroblasts / drug effects,  metabolism,  pathology
Osteopontin
Periodontium / drug effects*,  metabolism,  pathology
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Sialoglycoproteins / chemistry,  genetics,  metabolism*
Swine
Chemical
Reg. No./Substance:
0/Dental Enamel Proteins; 0/RNA, Messenger; 0/Sialoglycoproteins; 0/enamel matrix proteins; 106441-73-0/Osteopontin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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