Document Detail


Enhanced production of hydroperoxides by immobilized lipoxygenase in the presence of cyclodextrins.
MedLine Citation:
PMID:  16321060     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The advantages of the presence of cyclodextrins in a reaction catalyzed by immobilized lipoxygenase at neutral pH are reported for the first time. The steady-state rate in the presence of beta-cyclodextrins was seven times higher than in control experiments using the same concentration of linoleic acid; furthermore the percentage of substrate conversion (and product accumulation) obtained in the presence of beta-cyclodextrins was higher than in the control assays. The optimum concentration of free linoleic acid coincided with the critical micellar concentration for linoleic acid at neutral pH. The operational stability of the immobilized enzyme increased in the presence of beta-cyclodextins, while an increase in the percentage of 13-HPOD was also observed.
Authors:
Manuela P?rez-Gilabert; Francisco Garc?a-Carmona
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology progress     Volume:  21     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2005 Nov-Dec
Date Detail:
Created Date:  2005-12-02     Completed Date:  2006-02-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1742-7     Citation Subset:  IM    
Affiliation:
Departamento de Bioqu?mica y Biolog?a Molecular-A, Universidad de Murcia, Espinardo 30071, Spain. mpg@um.es
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MeSH Terms
Descriptor/Qualifier:
Biotechnology
Enzyme Stability
Enzymes, Immobilized
Kinetics
Lipid Peroxides / analysis,  biosynthesis*
Lipoxygenase / metabolism*
Polymers
Soybeans / enzymology
beta-Cyclodextrins
Chemical
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Lipid Peroxides; 0/Polymers; 0/beta-Cyclodextrins; 93356-75-3/Eupergit C; EC 1.13.11.12/Lipoxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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