| Enhanced production of hydroperoxides by immobilized lipoxygenase in the presence of cyclodextrins. | |
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MedLine Citation:
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PMID: 16321060 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The advantages of the presence of cyclodextrins in a reaction catalyzed by immobilized lipoxygenase at neutral pH are reported for the first time. The steady-state rate in the presence of beta-cyclodextrins was seven times higher than in control experiments using the same concentration of linoleic acid; furthermore the percentage of substrate conversion (and product accumulation) obtained in the presence of beta-cyclodextrins was higher than in the control assays. The optimum concentration of free linoleic acid coincided with the critical micellar concentration for linoleic acid at neutral pH. The operational stability of the immobilized enzyme increased in the presence of beta-cyclodextins, while an increase in the percentage of 13-HPOD was also observed. |
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Authors:
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Manuela P?rez-Gilabert; Francisco Garc?a-Carmona |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology progress Volume: 21 ISSN: 8756-7938 ISO Abbreviation: Biotechnol. Prog. Publication Date: 2005 Nov-Dec |
Date Detail:
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Created Date: 2005-12-02 Completed Date: 2006-02-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8506292 Medline TA: Biotechnol Prog Country: United States |
Other Details:
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Languages: eng Pagination: 1742-7 Citation Subset: IM |
Affiliation:
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Departamento de Bioqu?mica y Biolog?a Molecular-A, Universidad de Murcia, Espinardo 30071, Spain. mpg@um.es |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biotechnology Enzyme Stability Enzymes, Immobilized Kinetics Lipid Peroxides / analysis, biosynthesis* Lipoxygenase / metabolism* Polymers Soybeans / enzymology beta-Cyclodextrins |
| Chemical | |
Reg. No./Substance:
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0/Enzymes, Immobilized; 0/Lipid Peroxides; 0/Polymers; 0/beta-Cyclodextrins; 93356-75-3/Eupergit C; EC 1.13.11.12/Lipoxygenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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