| Enhanced pro-apoptotic and anti-adipogenic effects of genistein plus guggulsterone in 3T3-L1 adipocytes. | |
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MedLine Citation:
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PMID: 18525110 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Genistein (G), an isoflavone, and guggulsterone (GS), the active substance in guggulipid, have been reported to possess therapeutic effects for obesity. In the present study, we investigated the effects of combinations of G plus GS on apoptosis and adipogenesis in 3T3-L1 cells. In mature adipocytes, G and GS individually caused apoptosis, but combination of G plus GS significantly increased apoptosis, more than either compound alone. Furthermore, G plus GS caused a greater increase in procaspase-3 cleavage, Bax expression, cytochrome c release, and proteolytic cleavage of PARP than either compound alone. In maturing preadipocytes G and GS each suppressed lipid accumulation, but the combination potentiated the inhibition of lipid accumulation. These results suggest that combination of genistein and guggulsterone may exert anti-obesity effects by inhibiting adipogenesis and inducing apoptosis in adipocytes. |
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Authors:
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Jeong-Yeh Yang; Mary Anne Della-Fera; Srujana Rayalam; Suresh Ambati; Clifton A Baile |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: BioFactors (Oxford, England) Volume: 30 ISSN: 0951-6433 ISO Abbreviation: Biofactors Publication Date: 2007 |
Date Detail:
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Created Date: 2008-06-05 Completed Date: 2008-08-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8807441 Medline TA: Biofactors Country: Netherlands |
Other Details:
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Languages: eng Pagination: 159-69 Citation Subset: IM |
Affiliation:
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Department of Animal and Dairy Science, University of Georgia, Athens, GA 30602-2771, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3-L1 Cells Adipocytes / cytology, drug effects*, metabolism Adipogenesis / drug effects* Animals Apoptosis / drug effects* Blotting, Western Caspase 3 / metabolism Cell Survival / drug effects Cytochromes c / metabolism Drug Synergism Genistein / pharmacology* Lipid Metabolism / drug effects Mice Poly(ADP-ribose) Polymerases / metabolism Pregnenediones / pharmacology* bcl-2-Associated X Protein / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Pregnenediones; 0/bcl-2-Associated X Protein; 446-72-0/Genistein; 9007-43-6/Cytochromes c; 95975-55-6/pregna-4,17-diene-3,16-dione; EC 2.4.2.30/Parp1 protein, mouse; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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