Document Detail

Enhanced polymer one-step staining for proliferating cell nuclear antigen in squamous cell carcinoma of the tongue.
MedLine Citation:
PMID:  8957552     Owner:  NLM     Status:  MEDLINE    
A rapid enhanced polymer one-step staining for proliferating cell nuclear antigen (EPOS-PCNA) in formalin fixed, paraffin embedded sections of tongue squamous cell carcinoma is described. EPOS-PCNA was compared with PCNA immunohistochemistry using the avidin-biotin complex (ABC) method, with or without autoclave pretreatment. Significant correlation of PCNA labeling index (percentage of labeled cells/total cells counted) was observed between EPOS-PCNA and the immunohistochemical protocol using autoclave pretreatment. We consider this method a reliable, timesaving technique that would be useful in quantitative histopathology and in performing double immunostaining.
K Sano; J Sekine; T Inokuchi; M B Pe; G Ma
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  71     ISSN:  1052-0295     ISO Abbreviation:  Biotech Histochem     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-03-14     Completed Date:  1997-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  273-7     Citation Subset:  IM    
Second Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Carcinoma, Squamous Cell / immunology*,  pathology
Immunoenzyme Techniques
Proliferating Cell Nuclear Antigen / analysis*
Staining and Labeling / methods*
Tongue / immunology,  pathology
Tongue Neoplasms / immunology*,  pathology
Reg. No./Substance:
0/Polymers; 0/Proliferating Cell Nuclear Antigen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Angiotensin II type 1 receptor induced signal-transduction pathways as new targets for pharmacologic...
Next Document:  A comparison of methods for heat-mediated antigen retrieval for immunoelectron microscopy: demonstra...