Document Detail


Enhanced polymer one-step staining for proliferating cell nuclear antigen in squamous cell carcinoma of the tongue.
MedLine Citation:
PMID:  8957552     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A rapid enhanced polymer one-step staining for proliferating cell nuclear antigen (EPOS-PCNA) in formalin fixed, paraffin embedded sections of tongue squamous cell carcinoma is described. EPOS-PCNA was compared with PCNA immunohistochemistry using the avidin-biotin complex (ABC) method, with or without autoclave pretreatment. Significant correlation of PCNA labeling index (percentage of labeled cells/total cells counted) was observed between EPOS-PCNA and the immunohistochemical protocol using autoclave pretreatment. We consider this method a reliable, timesaving technique that would be useful in quantitative histopathology and in performing double immunostaining.
Authors:
K Sano; J Sekine; T Inokuchi; M B Pe; G Ma
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  71     ISSN:  1052-0295     ISO Abbreviation:  Biotech Histochem     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-03-14     Completed Date:  1997-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  273-7     Citation Subset:  IM    
Affiliation:
Second Department of Oral and Maxillofacial Surgery, Nagasaki University School of Dentistry, Japan.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Squamous Cell / immunology*,  pathology
Humans
Immunoenzyme Techniques
Polymers*
Proliferating Cell Nuclear Antigen / analysis*
Staining and Labeling / methods*
Tongue / immunology,  pathology
Tongue Neoplasms / immunology*,  pathology
Chemical
Reg. No./Substance:
0/Polymers; 0/Proliferating Cell Nuclear Antigen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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