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Enhanced photoacoustic detection of melanoma cells using gold nanoparticles.
MedLine Citation:
PMID:  21500228     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
BACKGROUND AND OBJECTIVES: We tagged melanoma cells with gold nanoparticles to show their viability for increasing sensitivity in a photoacoustic detection system. Ultimately, this study models the detection of circulating tumor cells, which are an important prognostic factor in the progress of melanoma.
STUDY DESIGN/MATERIALS AND METHODS: A Q-switched, tunable Nd:YAG laser was used to irradiate cells in both a stationary and flow set-up. Photoacoustic signals were measured using a polyvinylidene fluoride (PVDF) film in the stationary test, and a commercially available ultrasonic probe for flow tests. Both unmodified melanoma cells and gold nanoparticle (AuNP) tagged melanoma were tested.
RESULTS: AuNP tagged melanoma in a stationary set-up showed an average of 0.227 mV/mJ larger signal than the untagged, indicating a signal increase of 34%. At 500 nm there is a maximum difference of 0.295 mV/mJ, or a 41% increase. In flow tests, the ultrasound probe was able to detect single cells, but the increased signal from AuNP tagging was minimal.
CONCLUSION: AuNP tagging proved to give an increased photoacoustic signal allowing greater sensitivity in stationary metastasized melanoma detection systems using photoacoustics. Lasers Surg. Med. 43:333-338, 2011. © 2011 Wiley-Liss, Inc.
Authors:
Devin R McCormack; Kiran Bhattacharyya; Raghuraman Kannan; Kattesh Katti; John A Viator
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lasers in surgery and medicine     Volume:  43     ISSN:  1096-9101     ISO Abbreviation:  Lasers Surg Med     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8007168     Medline TA:  Lasers Surg Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  333-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Affiliation:
Department of Biological Engineering, University of Missouri, Columbia, Missouri.
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