| Enhanced phospholipase B activity and alteration of phospholipids and neutral lipids in Saccharomyces cerevisiae exposed to N-nitrosonornicotine. | |
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MedLine Citation:
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PMID: 21046464 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A tobacco-specific nitrosamine (TSNA), N-nitrosonornicotine (NNN), is a potent carcinogen present in cigarette smoke, and chronic exposure to it can lead to pulmonary cancer. NNN causes changes in phospholipid metabolism and the mechanism is yet to be elucidated. Exposure of Saccharomyces cerevisiae to 50 μM NNN leads to a substantial decrease in phosphatidylserine (PS) by 63%, phosphatidylcholine (PC) by 42% and phosphatidylethanolamine (PE) by 36% with a concomitant increase in lysophospholipids (LPL) by 25%. The alteration in phospholipid content was dependent on increasing NNN concentration. Reduced phospholipids were accompanied with increased neutral lipid content. Here we report for the first time that NNN exposure, significantly increases phospholipase B (PLB) activity and the preferred substrate is PC, a major phospholipid responsible for a series of metabolic functions. Furthermore, NNN also promotes the alteration of fatty acid (FA) composition; it increases the long chain fatty acid (C18 series) in phospholipids specifically phosphatidylethanolamine (PE) and PS; while on the contrary it increases short chain fatty acids in cardiolipin (CL). NNN mediated degradation of phospholipids is associated with enhanced PLB activity and alteration of phospholipid composition is accompanied with acyl chain remodelling. Understanding the altered phospholipid metabolism produced by NNN exposure is a worthwhile pursuit because it will help to understand the toxicity of tobacco smoke. |
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Authors:
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Panneerselvam Vijayaraj; Jayaraja Sabarirajan; Vasanthi Nachiappan |
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Publication Detail:
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Type: Journal Article Date: 2010-11-03 |
Journal Detail:
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Title: Antonie van Leeuwenhoek Volume: 99 ISSN: 1572-9699 ISO Abbreviation: Antonie Van Leeuwenhoek Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372625 Medline TA: Antonie Van Leeuwenhoek Country: Netherlands |
Other Details:
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Languages: eng Pagination: 567-77 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Bharathidasan University, Tiruchirappalli, 24, Tamilnadu, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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