| Enhanced phosphoinositide hydrolysis via overexpression of phospholipase C beta1 or delta1 inhibits stimulus-induced insulin release in insulinoma MIN6 cells. | |
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MedLine Citation:
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PMID: 9920735 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To study the effects of enhanced phosphoinositide hydrolysis on insulin secretion, phosphoinositide-specific phospholipase Cbeta1 (PLCbeta1) or PLCdelta1 was overexpressed in insulinoma MIN6 cells via adenoviral vectors. Inositol phosphate production stimulated by NaF (with AlCl3) in PLCbeta1-overexpressing cells and that stimulated by KCl or glucose in both PLCbeta1- and PLCdelta1-overexpressing cells were greater than that in control cells. In addition, reduced phosphatidylinositol-4,5-bisphosphate levels were observed in these cells stimulated by NaF or KCl. The greater phosphoinositide hydrolysis was accompanied by 25-45% inhibition of insulin secretion. These data suggest that excessive phosphoinositide hydrolysis inhibits secretagogue-induced insulin release in MIN6 cells. |
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Authors:
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H Ishihara; T Wada; N Kizuki; T Asano; Y Yazaki; M Kikuchi; Y Oka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 254 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1999 Jan |
Date Detail:
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Created Date: 1999-02-25 Completed Date: 1999-02-25 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 77-82 Citation Subset: IM |
Copyright Information:
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Copyright 1999 Academic Press. |
Affiliation:
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Third Department of Internal Medicine, University of Tokyo, Hongo, Tokyo, Bunkyo-ku, 113, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae Animals Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Genetic Vectors Hydrolysis Insulin / secretion* Insulinoma / genetics, metabolism* Isoenzymes / biosynthesis*, genetics Pancreatic Neoplasms / genetics, metabolism* Phosphatidylinositols / metabolism Phospholipase C beta Phospholipase C delta Transfection Tumor Cells, Cultured Type C Phospholipases / biosynthesis*, genetics |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 0/Phosphatidylinositols; 11061-68-0/Insulin; EC 3.1.4.-/Type C Phospholipases; EC 3.1.4.11/Phospholipase C beta; EC 3.1.4.11/Phospholipase C delta |
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