Document Detail

Enhanced phosphoinositide hydrolysis via overexpression of phospholipase C beta1 or delta1 inhibits stimulus-induced insulin release in insulinoma MIN6 cells.
MedLine Citation:
PMID:  9920735     Owner:  NLM     Status:  MEDLINE    
To study the effects of enhanced phosphoinositide hydrolysis on insulin secretion, phosphoinositide-specific phospholipase Cbeta1 (PLCbeta1) or PLCdelta1 was overexpressed in insulinoma MIN6 cells via adenoviral vectors. Inositol phosphate production stimulated by NaF (with AlCl3) in PLCbeta1-overexpressing cells and that stimulated by KCl or glucose in both PLCbeta1- and PLCdelta1-overexpressing cells were greater than that in control cells. In addition, reduced phosphatidylinositol-4,5-bisphosphate levels were observed in these cells stimulated by NaF or KCl. The greater phosphoinositide hydrolysis was accompanied by 25-45% inhibition of insulin secretion. These data suggest that excessive phosphoinositide hydrolysis inhibits secretagogue-induced insulin release in MIN6 cells.
H Ishihara; T Wada; N Kizuki; T Asano; Y Yazaki; M Kikuchi; Y Oka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  254     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-02-25     Completed Date:  1999-02-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  77-82     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Third Department of Internal Medicine, University of Tokyo, Hongo, Tokyo, Bunkyo-ku, 113, Japan.
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MeSH Terms
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Genetic Vectors
Insulin / secretion*
Insulinoma / genetics,  metabolism*
Isoenzymes / biosynthesis*,  genetics
Pancreatic Neoplasms / genetics,  metabolism*
Phosphatidylinositols / metabolism
Phospholipase C beta
Phospholipase C delta
Tumor Cells, Cultured
Type C Phospholipases / biosynthesis*,  genetics
Reg. No./Substance:
0/Isoenzymes; 0/Phosphatidylinositols; 11061-68-0/Insulin; EC 3.1.4.-/Type C Phospholipases; EC C beta; EC C delta

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