Document Detail


Enhanced motility of KGF-transfected breast cancer cells.
MedLine Citation:
PMID:  16619493     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: In a previous study, we reported that keratinocyte growth factor (KGF) produced a rapid increase in the motility of ER-positive breast cancer cells. Others have demonstrated that KGF treatment in rodent species produces rapid mammary ductal hyperplasia. Epithelial cells do not produce KGF; thus, in the present study, MCF-7 cells were stably transfected with a KGF-expressing vector and the motility and morphology of the transfected, non-transfected and empty vector cell lines compared. MATERIALS AND METHODS: A mammalian expression vector containing a KGF cDNA was transfected into MCF-7/beta cells, and two stable clones (MCF-7/beta/KGF-T8 and MCF-7/beta/KGF-T9) were identified. Western blotting of conditioned medium from these clones was used to confirm the expression of KGF. The motility of wild-type and KGF-transfected MCF-7 cells was compared using time-lapse videomicroscopy and a cell culture wounding model which examined cell migration over a period of 1-3 days. RESULTS: The Western blots demonstrated that the expression of KGF in both the MCF-7/beta/KGF-T8 and MCF-7/beta/KGF-T9 cell lines was higher than the wild-type and MCF-7/beta cell lines. The cell proliferation and migration distance was significantly greater for both KGF-transfected MCF-7 cell lines than the wild-type and MCF-7/beta cell lines under the same experimental conditions. Further, changes in motile morphology were observed in both the MCF-7/beta/KGF-T8 and MCF-7/beta/KGF-T9 cell lines. In addition, the MCF-7/beta/KGF-T8 clone was found to produce much larger tumors than both the MCF-7/beta/KGF-T9 and EV clones in mouse xenografts. These results indicated that autocrine production of KGF in the KGF-transfected MCF-7 cell lines enhanced cell migration, migration-related morphology and xenograft tumor growth. CONCLUSION: KGF-transfected MCF-7 cells displayed a much greater motility than non-transfected cells, confirming the KGF motility enhancement effect which we previously reported. The use of KGF-transfected breast cancer cells in the xenograft model may help to study the mechanism of KGF-mediated cell motility and to identify specific KGF antagonists that may be used to prevent or impede KGF-mediated metastatic progression.
Authors:
Xiao-Ping Zang; Elizabeth C Bullen; Sharmila Manjeshwar; Eldon R Jupe; Eric W Howard; J Thomas Pento
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Anticancer research     Volume:  26     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    2006 Mar-Apr
Date Detail:
Created Date:  2006-04-19     Completed Date:  2006-05-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  961-6     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Sciences, University of Oklahoma Health Sciences Center and InterGenetics Incorporated, Oklahoma City, OK 73117, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Breast Neoplasms / genetics,  metabolism,  pathology*,  secretion
Cell Line, Tumor
Cell Movement / physiology*
Fibroblast Growth Factor 7 / biosynthesis,  genetics,  physiology*,  secretion
Humans
Mice
Mice, Nude
Neoplasm Transplantation
Transfection
Transplantation, Heterologous
Grant Support
ID/Acronym/Agency:
CA-89740/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
126469-10-1/Fibroblast Growth Factor 7

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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