| Enhanced motility of KGF-transfected breast cancer cells. | |
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MedLine Citation:
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PMID: 16619493 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: In a previous study, we reported that keratinocyte growth factor (KGF) produced a rapid increase in the motility of ER-positive breast cancer cells. Others have demonstrated that KGF treatment in rodent species produces rapid mammary ductal hyperplasia. Epithelial cells do not produce KGF; thus, in the present study, MCF-7 cells were stably transfected with a KGF-expressing vector and the motility and morphology of the transfected, non-transfected and empty vector cell lines compared. MATERIALS AND METHODS: A mammalian expression vector containing a KGF cDNA was transfected into MCF-7/beta cells, and two stable clones (MCF-7/beta/KGF-T8 and MCF-7/beta/KGF-T9) were identified. Western blotting of conditioned medium from these clones was used to confirm the expression of KGF. The motility of wild-type and KGF-transfected MCF-7 cells was compared using time-lapse videomicroscopy and a cell culture wounding model which examined cell migration over a period of 1-3 days. RESULTS: The Western blots demonstrated that the expression of KGF in both the MCF-7/beta/KGF-T8 and MCF-7/beta/KGF-T9 cell lines was higher than the wild-type and MCF-7/beta cell lines. The cell proliferation and migration distance was significantly greater for both KGF-transfected MCF-7 cell lines than the wild-type and MCF-7/beta cell lines under the same experimental conditions. Further, changes in motile morphology were observed in both the MCF-7/beta/KGF-T8 and MCF-7/beta/KGF-T9 cell lines. In addition, the MCF-7/beta/KGF-T8 clone was found to produce much larger tumors than both the MCF-7/beta/KGF-T9 and EV clones in mouse xenografts. These results indicated that autocrine production of KGF in the KGF-transfected MCF-7 cell lines enhanced cell migration, migration-related morphology and xenograft tumor growth. CONCLUSION: KGF-transfected MCF-7 cells displayed a much greater motility than non-transfected cells, confirming the KGF motility enhancement effect which we previously reported. The use of KGF-transfected breast cancer cells in the xenograft model may help to study the mechanism of KGF-mediated cell motility and to identify specific KGF antagonists that may be used to prevent or impede KGF-mediated metastatic progression. |
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Authors:
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Xiao-Ping Zang; Elizabeth C Bullen; Sharmila Manjeshwar; Eldon R Jupe; Eric W Howard; J Thomas Pento |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Anticancer research Volume: 26 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 2006 Mar-Apr |
Date Detail:
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Created Date: 2006-04-19 Completed Date: 2006-05-11 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 961-6 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutical Sciences, University of Oklahoma Health Sciences Center and InterGenetics Incorporated, Oklahoma City, OK 73117, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Breast Neoplasms / genetics, metabolism, pathology*, secretion Cell Line, Tumor Cell Movement / physiology* Fibroblast Growth Factor 7 / biosynthesis, genetics, physiology*, secretion Humans Mice Mice, Nude Neoplasm Transplantation Transfection Transplantation, Heterologous |
| Grant Support | |
ID/Acronym/Agency:
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CA-89740/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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126469-10-1/Fibroblast Growth Factor 7 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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