Document Detail


Enhanced monoclonal antibody production by gradual increase of osmotic pressure.
MedLine Citation:
PMID:  19003334     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The time length required for the adaptation of AFP-27 hybridoma cells to high osmotic pressure and the effect of a gradual increase of osmotic pressure on monoclonal antibody production were investigated. When the cells were subjected to an increase of osmotic pressure from 300 mOsmol kg-1 to 366 mOsmol kg- 1, the intracellular content of osmoprotective free amino acids reached a maximum level 6 h after the osmotic pressure was increased to 366 mOsmol kg-1. The same time period of 6 h incubation at 366 mOsmol kg-1 was required to obtain a high growth rate of AFP-27 cells at 440 mOsmol kg-1 when the cells were subjected to a two-step increase of osmotic pressure from 300 mOsmol kg-1 to 366 mOsmol kg-1 and then to 440 mOsmol kg-1. The time length for the physiological adaptation of the cells to 366 mOsmol kg-1 was consequently estimated to be 6 h. Osmotic pressure during batch cultivation was gradually increased from 300 mOsmol kg-1 to 400 mOsmol kg-1 with an adaptation time of at least 6 h. The specific growth rates following a gradual increase of osmotic pressure were higher than those at a constant osmotic pressure of 400 mOsmol kg-1, while the specific monoclonal antibody production rate increased with the increase in the mean osmotic pressure. As a result, the cells grown under a gradual increase of osmotic pressure produced higher amounts of monoclonal antibodies than did those grown under constant osmotic pressure.
Authors:
J Lin; M Takagi; Y Qu; P Gao; T Yoshida
Related Documents :
7342844 - Colloids versus crystalloids as volume substitutes: clinical relevance of the serum onc...
8067874 - Alternative method of ultrafiltration after cardiopulmonary bypass.
17021804 - Comparison of the effects of mechanical and osmotic pressures on the collagen fiber arc...
2274734 - Evaluation of pancreatic tissue fluid pressure measurements intraoperatively and by son...
17392554 - Serum interleukin-6 correlates with endothelial dysfunction in healthy men independentl...
6143354 - Effects of adrenergic agonists and antagonists on the blood pressure and heart rate of ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytotechnology     Volume:  29     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  1999 Jan 
Date Detail:
Created Date:  2008-11-12     Completed Date:  2011-07-14     Revised Date:  2013-03-27    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  27-33     Citation Subset:  -    
Affiliation:
International Center for Biotechnology, Osaka University, 2-1 Yamada-oka, Suita-shi, Osaka, 565, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Influence of osmolarity and pH increase to achieve a reduction of monoclonal antibodies aggregates i...
Next Document:  Uptake and cell-killing activities of a series of Victoria blue derivatives in a mouse mammary tumou...