| Enhanced mobile hybridization of gold nanoparticles decorated with oligonucleotide in microchannel devices. | |
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MedLine Citation:
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PMID: 20689861 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mobile hybridization is a concept proposed and verified herein. We have designed a microfluidic device that is capable of enhancing passive mixing through the morphology of micro-structures, positioned along the channels of the device. We investigated the capability of these structures to promote mobile hybridization of fluorophore-labeled target oligonucleotides to oligonucleotide gold-nanoparticle (Au-NP) probes. This process is monitored with fluorescence through the quenching of the fluorescent signal within the device as the target oligonucleotides become bound to the Au-NP probes. We evaluated the fluorescent intensity of a sample image that showed enhanced probability of mobile hybridization of the samples, which was completed in about 7.2 s. Mobile hybridization is thus much more effective than traditional static hybridization (reaction overnight) limited by molecular diffusion. This approach promises an improved hybridization of samples with these probes, and is beneficial for microfluidic-based systems for biomedical detection. |
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Authors:
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Miao-Hsing Hsu; Wei-Feng Fang; Yu-Hsuan Lai; Jing-Tang Yang; Tsung-Lin Tsai; Dar-Bin Shieh |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-05 |
Journal Detail:
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Title: Lab on a chip Volume: 10 ISSN: 1473-0197 ISO Abbreviation: Lab Chip Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-07 Completed Date: 2010-11-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128948 Medline TA: Lab Chip Country: England |
Other Details:
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Languages: eng Pagination: 2583-7 Citation Subset: IM |
Affiliation:
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Department of Mechanical Engineering, National Taiwan University, Taipei, 10617, Taiwan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA
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analysis*,
chemistry*,
genetics Equipment Design Equipment Failure Analysis Gold / chemistry* Microfluidics / instrumentation* Nanostructures / chemistry* Nanotechnology / instrumentation* Oligonucleotide Array Sequence Analysis / instrumentation* |
| Chemical | |
Reg. No./Substance:
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7440-57-5/Gold; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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