Document Detail


Enhanced magnetic cell labeling efficiency using -NH2 coated MPIOs.
MedLine Citation:
PMID:  21446031     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Strategies have been developed for labeling cells with micron sized iron oxide particles (MPIOs) for in vivo visualization of cells by magnetic resonance imaging. Although this approach is well established and has a variety of applications, current protocols employ long labeling times. It has been previously demonstrated that incubation of dextran coated iron oxide nanoparticles with positively charged transfection agents, such as poly-L-lysine increases labeling efficiency. Therefore, we sought to ascertain whether preincubating MPIOs with various quantities of poly-L-lysine would similarly enhance the rate of magnetic cell labeling. This was also tested against an NH(2) functionalized, commercially available MPIO. Indeed, we demonstrate significantly increased rate of magnetic cell labeling with MPIOs previously incubated with varying amounts of poly-L-lysine, with robust intracellular labeling at 2 hours. Yet the most robust labeling was achieved with the MPIO-NH(2). Interestingly, even for particle formulations which still had negative zeta potential, enhancement of magnetic cell labeling was achieved.
Authors:
Kevin S Tang; Erik M Shapiro
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-03-28
Journal Detail:
Title:  Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine     Volume:  65     ISSN:  1522-2594     ISO Abbreviation:  Magn Reson Med     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-18     Completed Date:  2011-09-22     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  8505245     Medline TA:  Magn Reson Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1564-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Coculture Techniques
Contrast Media / pharmacology*
Dextrans / pharmacology
Ferric Compounds / pharmacology*
Fibroblasts / metabolism*
Flow Cytometry
Image Enhancement / methods
Lysine
Magnetic Resonance Imaging / methods*
Mice
Microscopy, Confocal
Nanoparticles
Particle Size
Grant Support
ID/Acronym/Agency:
DP2 OD004362/OD/NIH HHS; DP2 OD004362/OD/NIH HHS; DP2 OD004362-01/OD/NIH HHS; P30 NS052519/NS/NINDS NIH HHS; P30 NS052519/NS/NINDS NIH HHS; P30 NS052519-05/NS/NINDS NIH HHS; R21 NS065297/NS/NINDS NIH HHS; R21 NS065297/NS/NINDS NIH HHS; R21 NS065297-01/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Contrast Media; 0/Ferric Compounds; K3R6ZDH4DU/Dextrans; K3Z4F929H6/Lysine
Comments/Corrections

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