| Enhanced magnetic cell labeling efficiency using -NH2 coated MPIOs. | |
| | |
MedLine Citation:
|
PMID: 21446031 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Strategies have been developed for labeling cells with micron sized iron oxide particles (MPIOs) for in vivo visualization of cells by magnetic resonance imaging. Although this approach is well established and has a variety of applications, current protocols employ long labeling times. It has been previously demonstrated that incubation of dextran coated iron oxide nanoparticles with positively charged transfection agents, such as poly-L-lysine increases labeling efficiency. Therefore, we sought to ascertain whether preincubating MPIOs with various quantities of poly-L-lysine would similarly enhance the rate of magnetic cell labeling. This was also tested against an NH(2) functionalized, commercially available MPIO. Indeed, we demonstrate significantly increased rate of magnetic cell labeling with MPIOs previously incubated with varying amounts of poly-L-lysine, with robust intracellular labeling at 2 hours. Yet the most robust labeling was achieved with the MPIO-NH(2). Interestingly, even for particle formulations which still had negative zeta potential, enhancement of magnetic cell labeling was achieved. |
| | |
Authors:
|
Kevin S Tang; Erik M Shapiro |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-03-28 |
Journal Detail:
|
Title: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine Volume: 65 ISSN: 1522-2594 ISO Abbreviation: Magn Reson Med Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-05-18 Completed Date: 2011-09-22 Revised Date: 2012-09-19 |
Medline Journal Info:
|
Nlm Unique ID: 8505245 Medline TA: Magn Reson Med Country: United States |
Other Details:
|
Languages: eng Pagination: 1564-9 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 Wiley-Liss, Inc. |
Affiliation:
|
Department of Biomedical Engineering, Yale University, New Haven, Connecticut 06510, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Coculture Techniques Contrast Media / pharmacology* Dextrans / pharmacology Ferric Compounds / pharmacology* Fibroblasts / metabolism* Flow Cytometry Image Enhancement / methods Lysine Magnetic Resonance Imaging / methods* Mice Microscopy, Confocal Nanoparticles Particle Size |
| Grant Support | |
ID/Acronym/Agency:
|
DP2 OD004362/OD/NIH HHS; DP2 OD004362-01/OD/NIH HHS; P30 NS052519/NS/NINDS NIH HHS; P30 NS052519-05/NS/NINDS NIH HHS; R21 NS065297/NS/NINDS NIH HHS; R21 NS065297-01/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Contrast Media; 0/Ferric Compounds; 56-87-1/Lysine; 9004-54-0/Dextrans |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Constrained estimation of the arterial input function for myocardial perfusion cardiovascular magnet...
Next Document: Two-dimensional phase cycled reconstruction for inherent correction of echo-planar imaging Nyquist a...