| Enhanced laser thermal ablation for the in vitro treatment of liver cancer by specific delivery of multiwalled carbon nanotubes functionalized with human serum albumin. | |
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MedLine Citation:
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PMID: 21289990 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The main goal of this investigation was to develop and test a new method of treatment for human hepatocellular carcinoma (HCC). We present a method of carbon nanotube-enhanced laser thermal ablation of HepG2 cells (human hepatocellular liver carcinoma cell line) based on a simple multiwalled carbon nanotube (MWCNT) carrier system, such as human serum albumin (HSA), and demonstrate its selective therapeutic efficacy compared with normal hepatocyte cells. Both HepG2 cells and hepatocytes were treated with HSA-MWCNTs at various concentrations and at various incubation times and further irradiated using a 2 W, 808 nm laser beam. Transmission electron, phase contrast, and confocal microscopy combined with immunochemical staining were used to demonstrate the selective internalization of HSA-MWCNTs via Gp60 receptors and the caveolin-mediated endocytosis inside HepG2 cells. The postirradiation apoptotic rate of HepG2 cells treated with HSA-MWCNTs ranged from 88.24% (for 50 mg/L) at 60 sec to 92.34% (for 50 mg/L) at 30 min. Significantly lower necrotic rates were obtained when human hepatocytes were treated with HSA-MWCNTs in a similar manner. Our results clearly show that HSA-MWCNTs selectively attach on the albondin (aka Gp60) receptor located on the HepG2 membrane, followed by an uptake through a caveolin-dependent endocytosis process. These unique results may represent a major step in liver cancer treatment using nanolocalized thermal ablation by laser heating. |
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Authors:
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Cornel Iancu; Lucian Mocan; Constantin Bele; Anamaria Ioana Orza; Flaviu A Tabaran; Cornel Catoi; Rares Stiufiuc; Ariana Stir; Cristian Matea; Dana Iancu; Lucia Agoston-Coldea; Florin Zaharie; Teodora Mocan |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-01-17 |
Journal Detail:
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Title: International journal of nanomedicine Volume: 6 ISSN: 1178-2013 ISO Abbreviation: Int J Nanomedicine Publication Date: 2011 |
Date Detail:
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Created Date: 2011-02-03 Completed Date: 2011-06-06 Revised Date: 2011-07-25 |
Medline Journal Info:
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Nlm Unique ID: 101263847 Medline TA: Int J Nanomedicine Country: New Zealand |
Other Details:
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Languages: eng Pagination: 129-41 Citation Subset: IM |
Affiliation:
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Department of Nanomedicine, Iuliu Hatieganu University of Medicine and Pharmacy, Third Surgery Clinic, Cluj-Napoca, Romania. cornel.iancu@umfcluj.ro |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Caveolin 1
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metabolism Drug Delivery Systems / methods* Fluorescein-5-isothiocyanate Hep G2 Cells Humans Laser Therapy / methods* Liver Neoplasms / metabolism, pathology, therapy* Nanotubes, Carbon / chemistry* Necrosis Serum Albumin / administration & dosage*, chemistry, metabolism Sialoglycoproteins / metabolism Spectrophotometry, Ultraviolet Spectroscopy, Fourier Transform Infrared Statistics, Nonparametric |
| Chemical | |
Reg. No./Substance:
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0/CAV1 protein, human; 0/Caveolin 1; 0/Nanotubes, Carbon; 0/Serum Albumin; 0/Sialoglycoproteins; 0/sialoglycoprotein gp60; 3326-32-7/Fluorescein-5-isothiocyanate |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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