Document Detail


Enhanced labeling of mitotic retinal cells with an intraocular [3H]thymidine injection.
MedLine Citation:
PMID:  2259458     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tritiated thymidine ([3H]Tdr) was injected either intraocularly (i.o.) or intraperitoneally (i.p.). Autoradiography showed that more retinal cells were labeled by i.o. than by i.p. injection. Controls showed that the increased number of labeled retinal cells was not related to glioblast proliferation produced by trauma from the i.o. injection. After injection of identical doses, the peak concentration of [3H]Tdr available for incorporation into DNA was about 100 times greater after an i.o. than after an i.p. injection. The effective duration of the labeling pulse in goldfish was 9 times longer for an i.o. than an i.p. injection. This study shows that i.o. delivery of [3H]Tdr is preferable to i.p. injection for efficient delivery of label to proliferating retinal cells.
Authors:
A D Springer; E L Sabban; K D Morel; M F Berk; J R Vogel; B R Wilson
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroscience letters     Volume:  118     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1991-01-31     Completed Date:  1991-01-31     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-4     Citation Subset:  IM    
Affiliation:
Department of Cell Biology and Anatomy, New York Medical College, Valhalla 10595.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoradiography
Eye
Ganglia / cytology,  physiology
Goldfish
Injections
Injections, Intraperitoneal
Mitosis*
Retina / cytology*
Thymidine / diagnostic use
Grant Support
ID/Acronym/Agency:
EY-03552/EY/NEI NIH HHS; NS-01121/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
50-89-5/Thymidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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