Document Detail

Enhanced isoflurane suppression of excitatory synaptic transmission in the aged rat hippocampus.
MedLine Citation:
PMID:  9720776     Owner:  NLM     Status:  MEDLINE    
1. The effects of the volatile anaesthetic, isoflurane, were investigated on evoked dendritic field excitatory postsynaptic potentials (f.e.p.s.p.) and antidromic and orthodromic population spikes recorded extracellularly in the CA1 cell layer region in the in vitro hippocampal slice taken from young mature (2-3 months) and old (24-27 months) Fisher 344 rats. 2. Isoflurane depressed the and the orthodromically-evoked population spikes in both old and young hippocampi. However, the magnitude of the anaesthetic-induced depression was greater in slices taken from old rats compared to those taken from young rats during the application of different isoflurane concentrations (0.5-5%). 3. In the presence of the GABA(A) antagonist, bicuculline methiodide (15 microM), isoflurane suppressed the to the same extent as was observed in the absence of the GABA(A) antagonist. 4. Orthodromically evoked population spikes were suppressed by isoflurane in a manner quantitatively similar to the suppression of the However, antidromic population spikes and presynaptic volleys evoked in young and old slices were resistant to anaesthetic action. In addition, paired pulse facilitation ratio of the evoked dendritic was not affected in both young and old slices during the application of isoflurane. 5. When slices were exposed to low Ca2+/high Mg2+ solution, isoflurane (1 and 3%) depressed the in aged slices to the same extent as in young slices. 6. The augmented anaesthetic depression of in old compared to young hippocampi in the absence and presence of bicuculline, and the lack of anaesthetic effects on antidromic population spikes and presynaptic volleys in old and young slices, suggest that the increased sensitivity of anaesthetic actions in old hippocampi is due to age-induced attenuation of synaptic excitation rather than potentiation of synaptic inhibition. Furthermore, elimination of the increased sensitivity of old slices to anaesthetic actions when the slices were perfused with low Ca2+/high Mg2+ medium, which presumably would decrease intracellular [Ca2+], suggests that the enhanced anaesthetic effects in aged neurones might be related to increased intraneuronal [Ca2+] in the synaptic terminal.
A Ouanounou; P L Carlen; H El-Beheiry
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of pharmacology     Volume:  124     ISSN:  0007-1188     ISO Abbreviation:  Br. J. Pharmacol.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-10-26     Completed Date:  1998-10-26     Revised Date:  2008-11-20    
Medline Journal Info:
Nlm Unique ID:  7502536     Medline TA:  Br J Pharmacol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1075-82     Citation Subset:  IM    
Department of Medicine (Neurology), University of Toronto, The Toronto Hospital, Western Division, Ontario, Canada.
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MeSH Terms
Aging / physiology*
Bicuculline / pharmacology
Calcium / metabolism
Excitatory Postsynaptic Potentials
GABA Antagonists / pharmacology
Hippocampus / drug effects*,  physiology
Isoflurane / pharmacology*
Magnesium / metabolism
Rats, Inbred F344
Synaptic Transmission / drug effects*
Reg. No./Substance:
0/GABA Antagonists; 26675-46-7/Isoflurane; 485-49-4/Bicuculline; 7439-95-4/Magnesium; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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