| Enhanced intrinsic biomechanical properties of osteoblastic mineralized tissue on roughened titanium surface. | |
| | |
MedLine Citation:
|
PMID: 15654712 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The biological mechanisms underlying bone-titanium integration and biomechanical properties of the integrated bone are poorly understood. This study assesses intrinsic biomechanical properties of mineralized tissue cultured on titanium having different surface topographies. The osteoblastic phenotypes associated with mineral deposition and collagen synthesis underlying the biomechanical modulation are also reported. Rat bone marrow-derived osteoblastic cells were cultured either on the machined titanium disc or acid-etched titanium disc. Nano-indentation study of day 28 culture revealed that the mineralized tissue on the acid-etched surface shows 3-3.5 times greater hardness than that on the machined surface (p < 0.01). Elastic modulus of the mineralized tissue was also 2.5-3 times greater on the acid-etched surface than on the machined surface (p < 0.01). After 28 days of culture, mineralized nodule area was significantly lower on the acid-etched surface than on the machined surface (p = 0.0105), while total calcium deposition did not differ between the two surfaces, indicating denser mineral deposition on the acid-etched surface. Osteopontin and osteocalcin gene expressions assayed by the reverse transcriptase-polymerase chain reaction were upregulated in the acid-etched titanium culture. Collagen synthesis measured by Sirius red stain-based colorimetry was 1.5-10 times higher on the acid-etched surface than on the machined surface in the initial culture period of day 1 to day 14 (p < 0.0001). The amount of collagen synthesis corresponded with the enhanced gene expression of prolyl 4-hydroxylase, a key enzyme for post-translational modification of collagen chains. Scanning electron microscopic images revealed that tissue cultured on the acid-etched titanium exhibited plate-like, compact surface morphology, while the tissue on the machined titanium appeared porous and was covered by fibrous and punctate structures. We conclude that culturing osteoblasts on rougher titanium surfaces enhances hardness and elastic modulus of the mineralized tissue, associated with condensed mineralization, accelerated collagen synthesis, and upregulated expression of selected bone-related genes. |
| | |
Authors:
|
Kazuo Takeuchi; Lei Saruwatari; Hiromi K Nakamura; Jenn-Ming Yang; Takahiro Ogawa |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of biomedical materials research. Part A Volume: 72 ISSN: 1549-3296 ISO Abbreviation: - Publication Date: 2005 Mar |
Date Detail:
|
Created Date: 2005-02-07 Completed Date: 2005-06-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
|
Languages: eng Pagination: 296-305 Citation Subset: IM |
Affiliation:
|
The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, California 90095, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biocompatible Materials* Cell Proliferation Collagen / biosynthesis Gene Expression / physiology Male Microscopy, Electron, Scanning Osteoblasts / physiology* Rats Rats, Sprague-Dawley Titanium* |
| Chemical | |
Reg. No./Substance:
|
0/Biocompatible Materials; 7440-32-6/Titanium; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Experimental model for observation of micromotion in cell culture.
Next Document: A clinical, histological, and computer-based assessment of the Polaris LV, combination diode, and ra...