| Enhanced immunogenicity of peptide P277 by heat shock protein HSP65 vector carrying tandem repeats of P277 to prevent type 1 diabetes in NOD mice. | |
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MedLine Citation:
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PMID: 18473288 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The peptide P277 contains a target epitope for diabetogenic T cells and it has been used as an ideal target antigen to develop vaccines against type 1 diabetes. A major problem in developing P277 vaccine is its low immunogenicity. Recent applications involving multiple copies of self-peptide in linear alignment and conjugation with carrier proteins appear to increase the immune response. In this study, we designed a method based on isocaudamer technique to repeat tandemly the 24-residue sequence P277, then 6 tandemly repeated copies of the peptide P277 were fused to mycobacterial heat-shock protein 65 to construct a fusion protein HSP65-6xP277 as an immunogen. We examined the effect of the tandem repeats of the peptide P277 in eliciting an immune response by comparing the immunogenicity of the three immunogens: P277, HSP65-P277 and HSP65-6xP277. Immunization of mice with the fusion protein HSP65-6xP277 elicited much higher levels of specific anti-P277 antibodies than with P277 and HSP65-P277, which should suggest that multiple tandem repeats of a certain epitope is an efficient method to overcome the low immunogenicity of self-peptide antigens and the immunogen HSP65-6xP277 might be further developed to a vaccine against type 1 diabetes. |
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Authors:
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J Liang; Z Aihua; W Yu; L Jingjing |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-05-13 |
Journal Detail:
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Title: Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association Volume: 116 ISSN: 0947-7349 ISO Abbreviation: Exp. Clin. Endocrinol. Diabetes Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-11-21 Completed Date: 2009-06-19 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9505926 Medline TA: Exp Clin Endocrinol Diabetes Country: Germany |
Other Details:
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Languages: eng Pagination: 541-8 Citation Subset: IM |
Affiliation:
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Minigene Pharmacy Laboratory, Biopharmaceutical College, China Pharmaceutical University, Nanjing, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / immunology BCG Vaccine / immunology Bacterial Proteins / genetics, immunology* Chaperonin 60 Chaperonins / genetics, immunology* Diabetes Mellitus, Type 1 / immunology, pathology Epitopes / immunology Female Heat-Shock Proteins / chemistry, genetics, immunology*, therapeutic use Immunoassay Mice Mice, Inbred NOD Peptide Fragments / chemistry, genetics, immunology*, therapeutic use T-Lymphocytes / immunology* Vaccines |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/BCG Vaccine; 0/Bacterial Proteins; 0/Chaperonin 60; 0/Epitopes; 0/Heat-Shock Proteins; 0/Peptide Fragments; 0/Vaccines; 0/heat-shock protein 65, Mycobacterium; 0/peptide 277, heat shock protein 60; EC 3.6.1.-/Chaperonins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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