Document Detail


Enhanced immunocytochemical staining sensitivity in formalin-fixed and paraffin-embedded material by simultaneous use of PAP and ABC.
MedLine Citation:
PMID:  2079108     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The simultaneous use of two immunoperoxidase (IP) methods; e.g. the PAP (Peroxidase-antiperoxidase) and ABC (Avidin-Biotin) to localize a single antigen enhances the sensitivity of polyclonal antibodies (FVIII/RAg and laminin) or monoclonal antibodies (MABs) (against human B-, T-cells and macrophages) in routinely formalin fixed and in paraffin embedded material. The increased sensitivity was not accompanied by loss of specificity. With antibodies against Factor VIII related antigen (FVIII/RAg) and laminin the increased staining intensity of blood vessels were demonstrated in experimental rat transplantation tumors. The similar enhancement of immunocytochemical reactions was observed with biopsies of human brain tumors, where the monoclonal antibodies against white blood cells were used. The staining results were of comparable intensity as in snapfrozen tissue or after special embedding procedure for immunocytochemical purposes acc. to Bolton and Mesnard formol sucrose gum, sucrose paraffin; FSGSP).
Authors:
S Krajewski; R J Seitz; W Wechsler
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society     Volume:  28     ISSN:  0239-8508     ISO Abbreviation:  Folia Histochem. Cytobiol.     Publication Date:  1990  
Date Detail:
Created Date:  1991-05-02     Completed Date:  1991-05-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8502651     Medline TA:  Folia Histochem Cytobiol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  211-7     Citation Subset:  IM    
Affiliation:
Department of Neuropathology, Heinrich Heine University of Düsseldorf, Federal Republic of Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / diagnostic use
Antibodies, Monoclonal / diagnostic use,  immunology
Avidin / diagnostic use*
B-Lymphocytes / cytology,  immunology
Biotin / diagnostic use*
Brain Neoplasms / immunology,  pathology
Glioma / immunology,  pathology
Immunoenzyme Techniques
Immunohistochemistry / methods*
Laminin / immunology
Macrophages / cytology,  immunology
Neoplasm Transplantation
Peroxidases / diagnostic use*
Rats
T-Lymphocytes / cytology,  immunology
von Willebrand Factor / immunology
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antibodies, Monoclonal; 0/Laminin; 0/von Willebrand Factor; 1405-69-2/Avidin; 58-85-5/Biotin; EC 1.11.1.-/Peroxidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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