Document Detail


Enhanced human endothelial progenitor cell adhesion and differentiation by a bioinspired multifunctional nanomatrix.
MedLine Citation:
PMID:  23126402     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Endothelial progenitor cell (EPC) capturing techniques have led to revolutionary strategies that can improve the performance of cardiovascular implant devices and engineered tissues by enhancing re-endothelialization and angiogenesis. However, these strategies are limited by controversies regarding the phenotypic identities of EPCs as well as their inability to target and prevent the other afflictions associated with current therapies, namely thrombosis and neointimal hyperplasia. Therefore, the goal of this study is to study the efficacy of a bioinspired multifunctional nanomatrix in recruiting and promoting the differentiation of EPCs towards an endothelial lineage. The bioinspired nanomatrix combines multiple components including self-assembled peptide amphiphiles (PAs) that include cell adhesive ligands, nitric oxide (NO) producing donors, and enzyme mediated degradable sequences to achieve an endothelium mimicking character. In this study, human peripheral blood mononuclear cells (PBMNCs) were isolated and cultured on the bioinspired multifunctional nanomatrix. Initial cell adhesion, lectin staining, Ac-LDL uptake, and expression of endothelial markers including CD31, CD34, vWF, and VEGFR2 were analyzed. The results from this study indicate that the NO releasing bioinspired multifunctional nanomatrix promotes initial adhesion of EPCs when compared to control surfaces. The expression of endothelial markers is also increased on the bioinspired multifunctional nanomatrix, suggesting that it directs the differentiation of EPCs towards an endothelial phenotype. The bioinspired nanomatrix therefore provides a novel biomaterial based platform for capturing as well as directing EPC behavior. Therefore this study has the potential to positively impact the patency of cardiovascular devices such as stents and vascular grafts as well as enhanced angiogenesis for ischemic or engineered tissues.
Authors:
Adinarayana Andukuri; Young-Doug Sohn; Chidinma Anakwenze; Dong-Jin Lim; Brigitta Brott; Young-Sup Yoon; Ho-Wook Jun
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-6
Journal Detail:
Title:  Tissue engineering. Part C, Methods     Volume:  -     ISSN:  1937-3392     ISO Abbreviation:  Tissue Eng Part C Methods     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466663     Medline TA:  Tissue Eng Part C Methods     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
U of Alabama at Birmingham, Biomedical Engineering, Birmingham, Alabama, United States; andukuri@uab.edu.
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