Document Detail

Enhanced human endothelial progenitor cell adhesion and differentiation by a bioinspired multifunctional nanomatrix.
MedLine Citation:
PMID:  23126402     Owner:  NLM     Status:  MEDLINE    
Endothelial progenitor cell (EPC)-capturing techniques have led to revolutionary strategies that can improve the performance of cardiovascular implant devices and engineered tissues by enhancing re-endothelialization and angiogenesis. However, these strategies are limited by controversies regarding the phenotypic identities of EPCs as well as their inability to target and prevent the other afflictions associated with current therapies, namely, thrombosis and neointimal hyperplasia. Therefore, the goal of this study was to study the efficacy of a bioinspired multifunctional nanomatrix in recruiting and promoting the differentiation of EPCs toward an endothelial lineage. The bioinspired nanomatrix combines multiple components, including self-assembled peptide amphiphiles (PAs) that include cell adhesive ligands, nitric oxide (NO)-producing donors, and enzyme-mediated degradable sequences to achieve an endothelium-mimicking character. In this study, human peripheral blood mononuclear cells (PBMNCs) were isolated and cultured on the bioinspired multifunctional nanomatrix. Initial cell adhesion, lectin staining, acetylated low-density lipoprotein uptake, and expression of endothelial markers, including CD31, CD34, von Willebrand Factor, and VEGFR2, were analyzed. The results from this study indicate that the NO releasing bioinspired multifunctional nanomatrix promotes initial adhesion of EPCs when compared to control surfaces. The expression of endothelial markers is also increased on the bioinspired multifunctional nanomatrix, suggesting that it directs the differentiation of EPCs toward an endothelial phenotype. The bioinspired nanomatrix therefore provides a novel biomaterial-based platform for capturing as well as directing EPC behavior. Therefore, this study has the potential to positively impact the patency of cardiovascular devices such as stents and vascular grafts as well as enhanced angiogenesis for ischemic or engineered tissues.
Adinarayana Andukuri; Young-Doug Sohn; Chidinma P Anakwenze; Dong-Jin Lim; Brigitta C Brott; Young-Sup Yoon; Ho-Wook Jun
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-12-19
Journal Detail:
Title:  Tissue engineering. Part C, Methods     Volume:  19     ISSN:  1937-3392     ISO Abbreviation:  Tissue Eng Part C Methods     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-03-19     Completed Date:  2014-01-17     Revised Date:  2014-05-08    
Medline Journal Info:
Nlm Unique ID:  101466663     Medline TA:  Tissue Eng Part C Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  375-85     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Biocompatible Materials / pharmacology*
Biological Markers / metabolism
Cell Adhesion / drug effects
Cell Differentiation / drug effects*
Endothelial Cells / cytology*,  drug effects,  metabolism
Flow Cytometry
Molecular Sequence Data
Nanoparticles / chemistry*
Nitric Oxide / metabolism
Peptides / chemistry
Staining and Labeling
Stem Cells / cytology*,  drug effects,  metabolism
Tissue Scaffolds / chemistry*
Grant Support
10PRE3500024//PHS HHS; GM086256/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Biocompatible Materials; 0/Biological Markers; 0/Ligands; 0/Peptides; 31C4KY9ESH/Nitric Oxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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