| Enhanced heat-induced cellular leakage and death of Staphylococcus aureus and Salmonella typhimurium by 2,3 Butanedione. | |
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MedLine Citation:
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PMID: 12724923 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Influence of diacetyl on cellular leakage and/or death of Staphylococcus aureus (S. aureus; FRI-100) and Salmonella typhimurium (S. typhimurium) in the presence and absence of 30% sucrose and NZ-amine broth at 37, 45 and 55 degrees C was studied using Microbiological and Spectrophotmetric techniques. Diacetyl exists naturally in starter distillate and is usually added to enhance the flavor at the end of the fermentation process. Our objective was to examine its use before the onset of fermentation towards the safety of fermented products. Results showed significant difference (p < 0.05) between control and diacetyl treatments; average mean difference was > 5 log colony forming units per ml (CFU/ml) within 1 hour (h). Interesting trends were also observed when differences with respect to absorbance ratios when data was normalized. Results also showed that diacetyl (0.1%) caused more cellular leakage and death of S. aureus at 45 degrees C as compared to 37 degrees C, and that cellular leakage per se was not directly related to death. Diacetyl, however, caused rapid death of S. aureus and S. typhimurium in the presence of 30% sucrose and NZ-amine broth at 55 degrees C; > 7 log CFU/ml death within 1 and 2 h respectively. We also conducted a study with commercial starter distillate (7%) and proved its ability to control both organisms under the same test conditions within 1 h at 55 degrees C. It is concluded that diacetyl is a potential candidate for the control of foodborne pathogens especially under low water activity conditions such as those encountered during meat fermentation. |
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Authors:
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Ibrahim O Farah |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Biomedical sciences instrumentation Volume: 39 ISSN: 0067-8856 ISO Abbreviation: Biomed Sci Instrum Publication Date: 2003 |
Date Detail:
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Created Date: 2003-05-02 Completed Date: 2003-06-17 Revised Date: 2009-11-11 |
Medline Journal Info:
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Nlm Unique ID: 0140524 Medline TA: Biomed Sci Instrum Country: United States |
Other Details:
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Languages: eng Pagination: 377-82 Citation Subset: IM |
Affiliation:
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Department of Biology, Jackson State University, Jackson, MS 39217, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Division
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drug effects Cell Membrane Permeability / drug effects, physiology Cell Survival Cells, Cultured Colony Count, Microbial Diacetyl / pharmacology* Hot Temperature* Reference Values Reproducibility of Results Salmonella typhimurium / drug effects*, physiology Sensitivity and Specificity Staphylococcus aureus / drug effects*, physiology Temperature |
| Chemical | |
Reg. No./Substance:
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431-03-8/Diacetyl |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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