Document Detail


Enhanced heat-induced cellular leakage and death of Staphylococcus aureus and Salmonella typhimurium by 2,3 Butanedione.
MedLine Citation:
PMID:  12724923     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Influence of diacetyl on cellular leakage and/or death of Staphylococcus aureus (S. aureus; FRI-100) and Salmonella typhimurium (S. typhimurium) in the presence and absence of 30% sucrose and NZ-amine broth at 37, 45 and 55 degrees C was studied using Microbiological and Spectrophotmetric techniques. Diacetyl exists naturally in starter distillate and is usually added to enhance the flavor at the end of the fermentation process. Our objective was to examine its use before the onset of fermentation towards the safety of fermented products. Results showed significant difference (p < 0.05) between control and diacetyl treatments; average mean difference was > 5 log colony forming units per ml (CFU/ml) within 1 hour (h). Interesting trends were also observed when differences with respect to absorbance ratios when data was normalized. Results also showed that diacetyl (0.1%) caused more cellular leakage and death of S. aureus at 45 degrees C as compared to 37 degrees C, and that cellular leakage per se was not directly related to death. Diacetyl, however, caused rapid death of S. aureus and S. typhimurium in the presence of 30% sucrose and NZ-amine broth at 55 degrees C; > 7 log CFU/ml death within 1 and 2 h respectively. We also conducted a study with commercial starter distillate (7%) and proved its ability to control both organisms under the same test conditions within 1 h at 55 degrees C. It is concluded that diacetyl is a potential candidate for the control of foodborne pathogens especially under low water activity conditions such as those encountered during meat fermentation.
Authors:
Ibrahim O Farah
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biomedical sciences instrumentation     Volume:  39     ISSN:  0067-8856     ISO Abbreviation:  Biomed Sci Instrum     Publication Date:  2003  
Date Detail:
Created Date:  2003-05-02     Completed Date:  2003-06-17     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0140524     Medline TA:  Biomed Sci Instrum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  377-82     Citation Subset:  IM    
Affiliation:
Department of Biology, Jackson State University, Jackson, MS 39217, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Division / drug effects
Cell Membrane Permeability / drug effects,  physiology
Cell Survival
Cells, Cultured
Colony Count, Microbial
Diacetyl / pharmacology*
Hot Temperature*
Reference Values
Reproducibility of Results
Salmonella typhimurium / drug effects*,  physiology
Sensitivity and Specificity
Staphylococcus aureus / drug effects*,  physiology
Temperature
Chemical
Reg. No./Substance:
431-03-8/Diacetyl

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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