| Enhanced germicidal effects of pulsed UV-LED irradiation on biofilms. | |
| | |
MedLine Citation:
|
PMID: 20854456 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
AIMS: The major objective of the study was to evaluate the enhanced germicidal effects of low-frequency pulsed ultraviolet A (UVA)-light-emitting diode (LED) on biofilms. METHODS AND RESULTS: The germicidal effects of UVA-LED irradiation (365 nm, 0·28 mW cm(-2) , in pulsed or continuous mode) on Candida albicans or Escherichia coli biofilms were evaluated by determining colony-forming units. The morphological change of microbial cells in biofilms was observed using scanning electron microscopy. After 5-min irradiation, over 90% of viable micro-organisms in biofilms had been killed, and pulsed irradiation (1-1000 Hz) had significantly greater germicidal ability than continuous irradiation. Pulsed irradiation (100 Hz, 60 min) almost completely killed micro-organisms in biofilm (>99·9%), and 20-min irradiation greatly damaged both microbial species. Interestingly, few hyphae were found in irradiated Candida biofilms. Moreover, mannitol treatment, a scavenger of hydroxyl radicals (OH(•) ), significantly protected viable micro-organisms in biofilms from UVA-LED irradiation. CONCLUSIONS: The study demonstrated that pulsed UVA-LED irradiation has a strong germicidal effect (maximum at 100 Hz, over 5-min irradiation) and causes the disappearance of hyphal forms of Candida. SIGNIFICANCE AND IMPACT OF THE STUDY: This study can assist in developing a low-frequency pulsed UVA-LED system to be applied to pathogenic biofilms for disinfection. |
| | |
Authors:
|
J Li; K Hirota; H Yumoto; T Matsuo; Y Miyake; T Ichikawa |
Related Documents
:
|
945516 - Transient variations in the systolic pulsations in amplitude of intracranial echoes: th... 22622976 - A reliability study of light refractive tomography utilized for noninvasive measurement... 2262476 - Vagal cholinergic innervation of the airways in newborn cat and dog. 8090846 - The effects of selected stimulus waveforms on pulse and phase characteristics at sensor... 18063566 - Neural correlates of visually induced self-motion illusion in depth. 22427696 - The relative weight of shape and non-rigid motion cues in object perception: a model of... |
Publication Detail:
|
Type: Journal Article Date: 2010-09-21 |
Journal Detail:
|
Title: Journal of applied microbiology Volume: 109 ISSN: 1365-2672 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-11-12 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
|
Languages: eng Pagination: 2183-90 Citation Subset: IM |
Copyright Information:
|
© 2010 The Authors. Journal of Applied Microbiology © 2010 The Society for Applied Microbiology. |
Affiliation:
|
Department of Oral and Maxillofacial Prosthodontics and Oral Implantology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Hexavalent chromium reduction by bacterial consortia and pure strains from an alkaline industrial ef...
Next Document: Salmonella rarely detected in Mississippi coastal waters and sediment.