| Enhanced gene delivery using Bubble liposomes and ultrasound for folate-PEG liposomes. | |
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MedLine Citation:
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PMID: 22332850 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We have previously reported that the transfection efficiency of laminin-derived AG73-peptide labeled polyethyleneglycol-modified liposomes (AG73-PEG liposomes) was enhanced by echo-contrast gas entrapping PEG liposomes (Bubble liposomes, BLs) and ultrasound (US) exposure by improving endosomal escape. However, it has not been well understood whether BLs and US exposure can enhance the transfection efficiency of other carriers except AG73-PEG liposomes. In this study, to evaluate whether BLs and US exposure can be generally applied to gene delivery carriers, we focused on folate as a model ligand and examined whether BLs and US exposure could enhance the transfection efficiency of folate-PEG liposomes. Folate-PEG liposomes could internalize into cells efficiently, whereas they could not deliver genes into cytosol from endosomes sufficiently. BLs and US exposure could enhance the transfection efficiency of folate-PEG liposomes compared with folate-PEG liposomes alone without their direct induction into cells. These results suggested that BLs and US exposure could enhance the transfection efficiency of folate-PEG liposomes in the same manner as AG73-PEG liposomes. Thus, BLs and US exposure may be a promising tool to achieve efficient gene transfection into various gene carriers in general. |
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Authors:
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Daiki Omata; Yoichi Negishi; Shoko Hagiwara; Sho Yamamura; Yoko Endo-Takahashi; Ryo Suzuki; Kazuo Maruyama; Yukihiko Aramaki |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-2-15 |
Journal Detail:
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Title: Journal of drug targeting Volume: - ISSN: 1029-2330 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-2-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9312476 Medline TA: J Drug Target Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Drug Delivery and Molecular Biopharmaceutics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences , Hachioji, Tokyo , Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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