| Enhanced expressions and histological characteristics of intravenously administered plasmid DNA in rat lung. | |
| | |
| Jump to Full Text | |
MedLine Citation:
|
PMID: 11641524 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cationic liposome-mediated gene transfection is a promising method for gene therapy. In this study, the transfection efficiency and histological patterns were evaluated in rat lung after intravenous administration via femoral vein of naked plasmid DNA, naked plasmid DNA with pretreatment of DOTAP, and DOTAP-cholesterol-plasmid DNA complex. Plasmid DNA encoding bacterial LacZ gene was used. For quantification of LacZ gene expression, beta-galactosidase assay was performed. For histologic examination, X-gal staining and immunohistochemical staining for transfected gene products were performed. Pretreatment of DOTAP prior to the infusion of naked plasmid DNA increased transfection efficiency up to a level comparable to DOTAP-cholesterol-plasmid DNA complex injection. Transfected genes were mainly expressed in type II pneumocytes and alveolar macrophages in all animals. We conclude that the high transfection efficiency is achievable by intravenous administration of naked plasmid DNA with pretreatment of DOTAP, to a level comparable to DOTAP-cholesterol-plasmid DNA complex. In this regard, naked plasmid DNA administration with pretreatment of DOTAP could be a more feasible option for intravenous gene transfer than DOTAP-cholesterol-plasmid DNA complex, in that the former is technically easier and more cost-effective than the latter with a comparable efficacy, in terms of intravenous gene delivery to the lung. |
| | |
Authors:
|
S J Rha; Y P Wang |
Related Documents
:
|
12578554 - Preparation of small unilamellar vesicles (suv) and biophysical characterization of the... 18636404 - Complexes of cationic block copolymer micelles with dna: histone/dna complex mimetics. 11708884 - Adenovirus hexon protein enhances nuclear delivery and increases transgene expression o... 22561984 - Real-time pcr assay for universal detection and quantitation of all five species of fow... 7026974 - Initiation of chromosomal dna synthesis in vitro. 20669894 - Robust and photocontrollable dna capsules using azobenzenes. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of Korean medical science Volume: 16 ISSN: 1011-8934 ISO Abbreviation: J. Korean Med. Sci. Publication Date: 2001 Oct |
Date Detail:
|
Created Date: 2001-10-19 Completed Date: 2002-01-03 Revised Date: 2011-04-01 |
Medline Journal Info:
|
Nlm Unique ID: 8703518 Medline TA: J Korean Med Sci Country: Korea (South) |
Other Details:
|
Languages: eng Pagination: 567-72 Citation Subset: IM |
Affiliation:
|
Department of Thoracic & Cardiovascular Surgery, College of Medicine, Catholic University of Korea, Seoul, Korea. drrha@cmc.cuk.ac.kr |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals DNA / administration & dosage*, metabolism Galactosides / analysis Gene Therapy* Gene Transfer, Horizontal Immunohistochemistry Indoles / analysis Injections, Intravenous Lung / metabolism* Male Plasmids* Rats Rats, Inbred F344 Transfection* |
| Chemical | |
Reg. No./Substance:
|
0/Galactosides; 0/Indoles; 7240-90-6/5-bromo-4-chloro-3-indolyl beta-galactoside; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
|
Journal Information Journal ID (nlm-ta): J Korean Med Sci Journal ID (pmc): jkms ISSN: 1011-8934 ISSN: 1598-6357 Publisher: Korean Academy of Medical Sciences |
Article Information Download PDF  Print publication date: Month: 10 Year: 2001 Volume: 16 Issue: 5 First Page: 567 Last Page: 572 ID: 3057602 PubMed Id: 11641524 Publisher Item Identifier: 200110567 |
| Enhanced expressions and histological characteristics of intravenously administered plasmid DNA in rat lung. | |
| S. J. Rha | Email: drrha@cmc.cuk.ac.kr |
| Y. P. Wang | |
| Department of Thoracic & Cardiovascular Surgery, College of Medicine, Catholic University of Korea, Seoul, Korea. |
|
Article Categories:
|
|
Previous Document: Clinical characteristics of constrictive pericarditis diagnosed by echo-Doppler technique in Korea.
Next Document: Focal organizing pneumonia: CT and pathologic findings.