| Enhanced expression of receptor for advanced glycation end-products is associated with low circulating soluble isoforms of the receptor in Type 2 diabetes. | |
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MedLine Citation:
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PMID: 20726839 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The sRAGE [soluble RAGE (receptor for advanced glycation end-products)] lack the transmembrane and cytoplasmic domain of the full-length receptor and can function as a decoy for RAGE ligands. Recent evidence suggests that sRAGE may be a potential biomarker of RAGE-mediated pathology. The present study aimed to examine the relationship between RAGE expression in peripheral blood monocytes and circulating sRAGE and esRAGE (endogenous sRAGE, a splice variant of sRAGE) in Type 2 diabetes. Protein expression of RAGE and esRAGE in monocyte cell lysate was determined by Western blot in 53 diabetic patients and 52 controls. Monocyte cell-surface-bound full-length RAGE expression was measured using flow cytometry. Serum sRAGE, esRAGE and AGE (advanced glycation end products) were assayed by ELISA. The mean HbA1c (glycated haemoglobin) of the diabetic patients was 9.74% and serum AGEs was increased. Monocyte full-length RAGE expression was significantly higher in diabetic patients whereas esRAGE expression was reduced, and serum AGEs concentration was an independent determinant of monocyte cell surface full-length RAGE expression. Serum levels of sRAGE [573.3 (375.7-754.3) compared with 608.1 (405.3-940.8) pg/ml, P<0.05] and esRAGE [241.8 (154.6-356.6) compared with 286.5 (202.6-390.0) pg/ml, P<0.05; values are medians (interquartile range)] were decreased. There was an inverse association between monocyte RAGE expression and log(serum sRAGE) (r=-0.34, P=0.01) but not with esRAGE. In conclusion, despite an increase in full-length RAGE expression, esRAGE expression was down-regulated in the diabetic patients, and serum sRAGE and esRAGE was also reduced. Hence increased full-length RAGE levels are not associated with a similar increase in sRAGE isoforms levels. |
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Authors:
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Xystus H L Tam; Sammy W M Shiu; Lin Leng; Richard Bucala; D John Betteridge; Kathryn C B Tan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical science (London, England : 1979) Volume: 120 ISSN: 1470-8736 ISO Abbreviation: Clin. Sci. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-10-08 Completed Date: 2011-01-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7905731 Medline TA: Clin Sci (Lond) Country: England |
Other Details:
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Languages: eng Pagination: 81-9 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Hong Kong, Hong Kong, People's Republic of China. kcbtan@hkucc.hku.hk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western
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methods Cross-Sectional Studies Diabetes Mellitus, Type 2 / blood* Down-Regulation Female Glycosylation End Products, Advanced / blood Hemoglobin A, Glycosylated / metabolism Humans Male Middle Aged Monocytes / metabolism Protein Isoforms / blood Receptors, Immunologic / blood*, genetics |
| Chemical | |
Reg. No./Substance:
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0/Glycosylation End Products, Advanced; 0/Hemoglobin A, Glycosylated; 0/Protein Isoforms; 0/Receptors, Immunologic; 0/advanced glycosylation end-product receptor; 0/hemoglobin A1c protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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