Document Detail


Enhanced expression of nicotinamide N-methyltransferase in human papillary thyroid carcinoma cells.
MedLine Citation:
PMID:  14557485     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To gain an understanding of the molecular pathogenesis of thyroid cancer, we used DNA microarray to study the expression profiles of 10 different human thyroid carcinoma cell lines. These included papillary lines BHP 2-7, BHP 7-13, BHP 10-3, BHP 18-21, NPA 87, and TPC1; anaplastic lines ARO 81-1 and DRO 90-1; follicular line WRO 82-1; and medullary line HRO 85-1. Among the genes with increased expression in the cancer cell lines, a gene coding for nicotinamide N-methyltransferase (NNMT) was identified for being highly expressed only in the papillary cell lines. NNMT catalyzes N-methylation of nicotinamide and other structurally related compounds and is highly expressed in the human liver. The results were further confirmed by semiquantitative RT-PCR and Northern blot analysis. NNMT catalytic activities were determined in all of the cells described above and in additional cell lines. Significantly higher NNMT enzyme activities were detected in eight of 10 of the papillary lines and three of six of the follicular cell lines tested. Normal thyroid tissue, thyroid primary cultures, anaplastic cancer cells, and medullary cancer cells showed no or low enzyme activity. Immunohistochemical staining for NNMT of human thyroid specimens showed strong and abundant cytoplasmic reactions in the sections of papillary carcinomas, and weak or scanty reaction in the normal thyroid tissues. These results indicate that NNMT is a potential biomarker for papillary thyroid carcinoma.
Authors:
Jimin Xu; Farhad Moatamed; Jeremy S Caldwell; John R Walker; Zaki Kraiem; Katsumi Taki; Gregory A Brent; Jerome M Hershman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  88     ISSN:  0021-972X     ISO Abbreviation:  J. Clin. Endocrinol. Metab.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-14     Completed Date:  2003-11-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4990-6     Citation Subset:  AIM; IM    
Affiliation:
Endocrinology and Diabetes Division, Veterans Affairs Medical Center, University of California School of Medicine, Los Angeles, California 90073, USA.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Northern
Carcinoma, Medullary / enzymology,  pathology
Carcinoma, Papillary, Follicular / enzymology*,  pathology*
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Humans
Immunohistochemistry
Methyltransferases / genetics*,  metabolism
Nicotinamide N-Methyltransferase
Oligonucleotide Array Sequence Analysis
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Thyroid Neoplasms / enzymology*,  pathology*
Tumor Cells, Cultured
Tumor Markers, Biological
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/Tumor Markers, Biological; EC 2.1.1.-/Methyltransferases; EC 2.1.1.1/NNMT protein, human; EC 2.1.1.1/Nicotinamide N-Methyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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