Document Detail

Enhanced expression of neurotrophic factors by C6 rat glioma cells after transfection with glia maturation factor.
MedLine Citation:
PMID:  10327166     Owner:  NLM     Status:  MEDLINE    
Glia maturation factor (GMF) is a 17-kDa protein unique to the nervous system. Although GMF was initially characterized as a growth/differentiation factor, the absence of a leader sequence and its intracellular localization in normal brain suggest an intracellularfunction as well. In this paper we transfected the C6 glioma cells with GMF cDNA by infecting the cells with a GMF/adenovirus construct. The transfected cells overexpressed GMF but did not secret the protein into the culture medium. However, the transfected cells showed an increased expression of the neurotrophic factors including nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF). The increase in neurotrophic activity of the C6 cell conditioned medium was demonstrable by its ability to promote neurite outgrowth in PC12 cells.
A Zaheer; J L Weiss; P Goyal; R Lim
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neuroscience letters     Volume:  265     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-07-01     Completed Date:  1999-07-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  203-6     Citation Subset:  IM    
Department of Neurology, University of Iowa College of Medicine and Veterans Affairs Medical Center, Iowa City 52242, USA.
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MeSH Terms
Adenoviridae / genetics
Brain-Derived Neurotrophic Factor / genetics*
Culture Media, Conditioned / pharmacology
Gene Expression Regulation, Neoplastic
Gene Expression Regulation, Viral
Glia Maturation Factor
Growth Inhibitors / genetics*
Lac Operon
Nerve Growth Factors / genetics*
Nerve Tissue Proteins / genetics*
Neurites / physiology
Neurons / cytology,  ultrastructure
PC12 Cells
RNA, Messenger / analysis
Recombinant Fusion Proteins / genetics
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured / drug effects,  physiology
Grant Support
Reg. No./Substance:
0/Brain-Derived Neurotrophic Factor; 0/Culture Media, Conditioned; 0/Glia Maturation Factor; 0/Growth Inhibitors; 0/Nerve Growth Factors; 0/Nerve Tissue Proteins; 0/RNA, Messenger; 0/Recombinant Fusion Proteins

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