Document Detail


Enhanced expression of glucose-6-phosphate dehydrogenase in human cells sustaining oxidative stress.
MedLine Citation:
PMID:  9169615     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent reports have demonstrated that glucose-6-phosphate dehydrogenase (G6PD) activity in mammalian cells is necessary in order to ensure cell survival when damage is produced by reactive oxygen intermediates. In this paper we demonstrate that oxidative stress, caused by agents acting at different steps in the biochemical pathway controlling the intracellular redox status, determines the increase in G6PD-specific activity in human cell lines of different tissue origins. The intracellular level of G6PD-specific mRNA also increases, with kinetics compatible with the induction of new enzyme synthesis. We carried out experiments in which cells were exposed to oxidative stress in the presence of inhibitors of protein or RNA synthesis. These demonstrated that increased G6PD expression is mainly due to an increased rate of transcription, with a minor but significant contribution of regulatory mechanisms acting at post-transcriptional levels. These results provide new information on the defence systems that eukaryotic cells possess in order to prevent damage caused by potentially harmful oxygen derivatives.
Authors:
M V Ursini; A Parrella; G Rosa; S Salzano; G Martini
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  323 ( Pt 3)     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1997 May 
Date Detail:
Created Date:  1997-06-27     Completed Date:  1997-06-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  801-6     Citation Subset:  IM    
Affiliation:
Istituto Internazionale di Genetica e Biofisica, Consiglio Nazionale delle Ricerche, Via Guglielmo Marconi 12, 80125 Naples, Italy.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Hepatocellular / pathology
Catalase / metabolism
Cycloheximide / pharmacology
Dactinomycin / pharmacology
Diamide / pharmacology
Enzyme Induction
Glucosephosphate Dehydrogenase / biosynthesis*,  genetics
Glutathione / metabolism
Humans
Hydrogen Peroxide / pharmacology
Leukemia-Lymphoma, Adult T-Cell / pathology
Liver Neoplasms / pathology
Neoplasm Proteins / biosynthesis,  genetics
Nucleic Acid Synthesis Inhibitors / pharmacology
Oxidation-Reduction
Oxidative Stress*
Protein Synthesis Inhibitors / pharmacology
RNA, Messenger / biosynthesis,  genetics
RNA, Neoplasm / biosynthesis,  genetics
Tumor Cells, Cultured / drug effects
Chemical
Reg. No./Substance:
0/Neoplasm Proteins; 0/Nucleic Acid Synthesis Inhibitors; 0/Protein Synthesis Inhibitors; 0/RNA, Messenger; 0/RNA, Neoplasm; 10465-78-8/Diamide; 50-76-0/Dactinomycin; 66-81-9/Cycloheximide; 70-18-8/Glutathione; 7722-84-1/Hydrogen Peroxide; EC 1.1.1.49/Glucosephosphate Dehydrogenase; EC 1.11.1.6/Catalase
Comments/Corrections

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