| Enhanced expression of glucose-6-phosphate dehydrogenase in human cells sustaining oxidative stress. | |
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MedLine Citation:
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PMID: 9169615 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent reports have demonstrated that glucose-6-phosphate dehydrogenase (G6PD) activity in mammalian cells is necessary in order to ensure cell survival when damage is produced by reactive oxygen intermediates. In this paper we demonstrate that oxidative stress, caused by agents acting at different steps in the biochemical pathway controlling the intracellular redox status, determines the increase in G6PD-specific activity in human cell lines of different tissue origins. The intracellular level of G6PD-specific mRNA also increases, with kinetics compatible with the induction of new enzyme synthesis. We carried out experiments in which cells were exposed to oxidative stress in the presence of inhibitors of protein or RNA synthesis. These demonstrated that increased G6PD expression is mainly due to an increased rate of transcription, with a minor but significant contribution of regulatory mechanisms acting at post-transcriptional levels. These results provide new information on the defence systems that eukaryotic cells possess in order to prevent damage caused by potentially harmful oxygen derivatives. |
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Authors:
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M V Ursini; A Parrella; G Rosa; S Salzano; G Martini |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 323 ( Pt 3) ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1997 May |
Date Detail:
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Created Date: 1997-06-27 Completed Date: 1997-06-27 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 801-6 Citation Subset: IM |
Affiliation:
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Istituto Internazionale di Genetica e Biofisica, Consiglio Nazionale delle Ricerche, Via Guglielmo Marconi 12, 80125 Naples, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Hepatocellular
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pathology Catalase / metabolism Cycloheximide / pharmacology Dactinomycin / pharmacology Diamide / pharmacology Enzyme Induction Glucosephosphate Dehydrogenase / biosynthesis*, genetics Glutathione / metabolism Humans Hydrogen Peroxide / pharmacology Leukemia-Lymphoma, Adult T-Cell / pathology Liver Neoplasms / pathology Neoplasm Proteins / biosynthesis, genetics Nucleic Acid Synthesis Inhibitors / pharmacology Oxidation-Reduction Oxidative Stress* Protein Synthesis Inhibitors / pharmacology RNA, Messenger / biosynthesis, genetics RNA, Neoplasm / biosynthesis, genetics Tumor Cells, Cultured / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Neoplasm Proteins; 0/Nucleic Acid Synthesis Inhibitors; 0/Protein Synthesis Inhibitors; 0/RNA, Messenger; 0/RNA, Neoplasm; 10465-78-8/Diamide; 50-76-0/Dactinomycin; 66-81-9/Cycloheximide; 70-18-8/Glutathione; 7722-84-1/Hydrogen Peroxide; EC 1.1.1.49/Glucosephosphate Dehydrogenase; EC 1.11.1.6/Catalase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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