Document Detail

Enhanced expression of Iba1, ionized calcium-binding adapter molecule 1, after transient focal cerebral ischemia in rat brain.
MedLine Citation:
PMID:  11340235     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND PURPOSE: Iba1 is a novel calcium-binding protein and is specifically expressed in microglia in the brain. It has been suggested that Iba1 plays an important role in regulation of the function of microglia. In the present study we examined time-dependent Iba1 expression after transient middle cerebral artery occlusion and characterized microglial activation in various brain regions. METHODS: Rat middle cerebral artery occlusion was induced by the intraluminal filament technique. After 1.5 hours of transient ischemia, Iba1 expression was examined by immunohistochemical and immunoblot analyses. The microglial activation in association with ischemic severity was characterized by double immunostaining with other specific markers. RESULTS: In the peri-ischemic area, heavily Iba1 immunoreactive cells rapidly appeared at 3.5 hours after reperfusion. Immunoreactivity was further increased and peaked at 7 days. In the ischemic core, round Iba1-positive cells, which may be blood-borne monocytes, appeared from 24 hours and reached a peak at 4 to 7 days. Double immunostaining revealed that activated microglia in the peri-ischemic area upregulated Iba1 expression but were negative for the macrophage marker ED1. ED1-positive cells were clearly restricted to the ischemic core. CONCLUSIONS: These findings suggest the following: (1) Iba1 expression may be associated with microglial activation in ischemic brain, and Iba1 immunostaining can be useful to evaluate the pathophysiological roles of activated microglia in ischemic injury. (2) Expression of ED1 antigen is strictly restricted to severe ischemic damage, whereas activated microglia in the peri-ischemic area showed Iba1 upregulation without ED1. Therefore, microglia may exhibit difference of antigenicity in the severity of ischemic brain injury.
D Ito; K Tanaka; S Suzuki; T Dembo; Y Fukuuchi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stroke; a journal of cerebral circulation     Volume:  32     ISSN:  1524-4628     ISO Abbreviation:  Stroke     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-09-06     Completed Date:  2001-09-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0235266     Medline TA:  Stroke     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1208-15     Citation Subset:  IM    
Department of Neurology, School of Medicine, Keio University, Tokyo, Japan.
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MeSH Terms
Antigens, Differentiation / metabolism
Brain / blood supply*,  metabolism*,  pathology
Calcium-Binding Proteins / biosynthesis*
Disease Models, Animal
Ischemic Attack, Transient / metabolism*,  pathology
Macrophages / metabolism
Microglia / metabolism
Rats, Sprague-Dawley
Time Factors
Reg. No./Substance:
0/Aif1 protein, rat; 0/Antigens, Differentiation; 0/Calcium-Binding Proteins

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