| Enhanced endothelin synthesis by endothelial cells exposed to sera from pregnant rats with decreased uterine perfusion. | |
| | |
MedLine Citation:
|
PMID: 16391174 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The initiating event in preeclampsia is thought be to reduced uteroplacental perfusion. Although we have reported previously that chronic reductions in uterine perfusion pressure (RUPP) in pregnant rats results in hypertension and enhanced endothelin production, the factors linking placental ischemia and endothelial cell activation remain unclear. The purpose of this study was to determine the role of angiotensin II type-1 (AT1) receptor activation on endothelin production induced by serum from pregnant rats exposed to reductions in uterine perfusion. To achieve this goal, human umbilical vein endothelial cells were exposed to sera collected from RUPP rats or normal pregnant rats. Arterial pressure was significantly higher in RUPP rats (135+/-2 mm Hg) than in pregnant rats (106+/-1 mm Hg). Six hours after exposure to RUPP serum (n=17), cell media endothelin concentration was 18.4+/-2.7 pg/mL as compared with 9.22+/-1.3 pg/mL from cells exposed to serum from normal pregnant rats (n=9). Eighteen hours after exposure to RUPP serum (n=7), endothelin concentration was 30.5+/-3.8 pg/mL as compared with 12.8+/-5.3 pg/mL from cells exposed to normal pregnant rat serum (n=6). In contrast, serum from RUPP rats did not increase endothelin production in human umbilical vein endothelial cells pretreated with an AT1 receptor antagonist, losartan (15 micromol/L). Eighteen hours after exposure to RUPP serum and losartan (n=14), endothelin concentration was 21.3+/-2.2 pg/mL as compared with 16.4+/-3.3 pg/mL from cells exposed to normal pregnant rat serum and losartan (n=10). These data indicate that serum from pregnant rats exposed to reductions in uterine perfusion enhances endothelin production by endothelial cells via by AT1 receptor activation. |
| | |
Authors:
|
Lyndsay Roberts; B Babbette D LaMarca; Lillian Fournier; Jennifer Bain; Kathy Cockrell; Joey P Granger |
Related Documents
:
|
180034 - Metabolism of 125i-labeled lipoproteins by the isolated rat lung. 19288164 - Mechanism for myocardial localization and rapid liver clearance of tc-99m-n-mpo: a new ... 7749654 - Stevioside is not metabolized in the isolated perfused rat liver. 7752844 - Uptake and distribution of exogenous coq in the mitochondrial fraction of perfused rat ... 9610374 - Increased plasma p-selectin induced by intravenous administration of endotoxin in rats. 215794 - Localization of prolyl hydroxylase by the immunoperoxidase method in cardiovascular tis... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2006-01-03 |
Journal Detail:
|
Title: Hypertension Volume: 47 ISSN: 1524-4563 ISO Abbreviation: Hypertension Publication Date: 2006 Mar |
Date Detail:
|
Created Date: 2006-02-17 Completed Date: 2006-03-17 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 7906255 Medline TA: Hypertension Country: United States |
Other Details:
|
Languages: eng Pagination: 615-8 Citation Subset: IM |
Affiliation:
|
Department of Physiology and Biophysics, Center for Excellence in Cardiovascular-Renal Research, University of Mississippi Medical Center, Jackson, MS 39216-4505, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Angiotensin II Type 1 Receptor Blockers
/
pharmacology Animals Cells, Cultured Endothelial Cells / drug effects, metabolism* Endothelins / biosynthesis*, metabolism Female Humans Losartan / pharmacology Osmolar Concentration Pregnancy Pregnancy, Animal / blood* Rats Rats, Sprague-Dawley Receptor, Angiotensin, Type 1 / metabolism* Regional Blood Flow Time Factors Uterus / blood supply* |
| Grant Support | |
ID/Acronym/Agency:
|
HL38499/HL/NHLBI NIH HHS; HL51971/HL/NHLBI NIH HHS; HL66661/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Angiotensin II Type 1 Receptor Blockers; 0/Endothelins; 0/Receptor, Angiotensin, Type 1; 114798-26-4/Losartan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Vasorelaxing effect of BAY 41-2272 in rat basilar artery: involvement of cGMP-dependent and independ...
Next Document: Chromosome 2p shows significant linkage to antihypertensive response in the British Genetics of Hype...