Document Detail

Enhanced differentiation of mesenchymal stem cells into NP-like cells via 3D co-culturing with mechanical stimulation.
MedLine Citation:
PMID:  19577195     Owner:  NLM     Status:  MEDLINE    
This study proposes a three-dimensional co-culturing system of mesenchymal stem cells (MSCs) and nucleus pulposus (NP) cells from New Zealand white male rabbits to differentiate MSCs into NP-like cells. The preferable ratio of MSCs to NP cells and the effects of mechanical stimulation were investigated without biochemical reagents. The preferable ratio was investigated without mechanical stimulation using five groups: Group I (MSC control); Group II (NP cell control); and Groups III, IV, and V, for which the ratios of NP cells to MSCs were 1:1, 1:2, and 2:1, respectively. During culture for 10 days without stimulation, the proliferation of MSCs did not increase after day 4. NP cells proliferated more when co-cultured as in Group V. However, the degree of differentiation of MSCs increased significantly in Group V. The differentiation of NP cells decreased gradually over time. When mechanical stimulation was applied to Groups I, II, and V, it contributed to the differentiation of MSCs into NP-like cells, as well as to that of NP cells, but did not contribute to the proliferation of either cell type. The contribution of mechanical stimulation to differentiation was also confirmed by RT-PCR.
Dong Hwa Kim; Su-Hyang Kim; Su-Jin Heo; Ji Won Shin; Shi Woo Lee; Su A Park; Jung-Woog Shin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  108     ISSN:  1347-4421     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-06     Completed Date:  2009-11-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  63-7     Citation Subset:  IM    
National Research Laboratory of Cellular and Biomechanical Engineering, Department of Biomedical Engineering, Inje University, Gimhae, Gyeongnam 621-749, Republic of Korea.
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MeSH Terms
Base Sequence
Cell Differentiation*
Coculture Techniques
DNA Primers
Mesenchymal Stem Cells / cytology*
Polymerase Chain Reaction
Stress, Mechanical*
Reg. No./Substance:
0/DNA Primers

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