Document Detail

Enhanced detection of cardiac myocyte damage by polarized light microscopy. Use in a model of coxsackievirus B3-induced myocarditis.
MedLine Citation:
PMID:  11425601     Owner:  NLM     Status:  MEDLINE    
Although accurate detection of cardiac muscle damage is critical in the diagnosis of acute myocarditis or acute cellular rejection in both clinical and experimental settings, the histologic evaluation is frequently uncertain without specialized stains. In a study of adult male A/J mice infected with 2x10(5) plaque-forming units of myocarditic coxsackievirus B3, cardiac muscle injury causing myofibrillar disruption was detected as a loss of muscle birefringence by polarized light microscopy. The technique was corroborated by comparison with Masson's trichrome stain and was helpful for histologic examination especially at the early preinflammatory stages of lesion development or in fringe territories of focal lesions. Polarized light microscopy is thus an available means to enhance the histologic determination of cardiac myocyte damage and has specific advantage in an absence of specialized stains.
L H Chow; D R Boughner; J Dunmore-Buyze; H Finlay; J G Pickering
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology     Volume:  10     ISSN:  1054-8807     ISO Abbreviation:  Cardiovasc. Pathol.     Publication Date:    2001 Mar-Apr
Date Detail:
Created Date:  2001-06-26     Completed Date:  2001-08-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9212060     Medline TA:  Cardiovasc Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  83-6     Citation Subset:  IM    
Vascular Biology Group, John P. Robarts Research Institute, London, Ontario, Canada.
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MeSH Terms
Coxsackievirus Infections / diagnosis*,  etiology
Disease Models, Animal
Enterovirus B, Human / physiology
Mice, Inbred A
Microscopy, Polarization*
Myocarditis / diagnosis*,  virology
Myocardium / pathology*
Myofibrils / pathology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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