Document Detail


Enhanced cytokines delivery and intercellular adhesion molecule 1 (ICAM-1) expression in glioma by intracarotid infusion of bradykinin analog, RMP-7.
MedLine Citation:
PMID:  9329027     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of intracarotid infusion of the bradykinin analog, RMP-7, on blood-to-tumor and blood-to-brain transport of three cytokines were investigated. Wistar rats with RG2 gliomas were utilized and a unidirectional transfer constant, Ki, was determined using quantitative autoradiography. Interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), and interleukin-2 (IL-2) were labeled with 125Iodine for quantitative transport studies using autoradiography. Radiolabeled cytokines were injected as an intravenous bolus. Intracarotid infusion of RMP-7 (0.1 microgram kg-1 min-1) increased the selective transport to tumors of IFN-gamma by 3.97-fold (p < 0.005), of TNF-alpha by 5.30-fold (p < 0.005), and of IL-2 by 4.34-fold (p < 0.005), compared to intracarotid saline infusion. To determine whether the increased IFN-gamma or TNF-alpha transport to tumors with RMP-7 could enhance expression of intercellular adhesion molecule 1 (ICAM-1) in tumors, ICAM-1 expression in RG2 glioma was evaluated by immunohistochemistry. Both IFN-gamma and TNF-alpha increased ICAM-1 expression of RG2 cells in vitro. In vivo, intracarotid infusion of IFN-gamma combined with RMP-7 significantly enhanced ICAM-1 expression in intracerebral RG2 gliomas compared to infusion of IFN-gamma without RMP-7. Expression of ICAM-1 was not enhanced by TNF-alpha combined with RMP-7. Intracarotid infusion of RMP-7 is a novel method of cytokines delivery to brain tumors.
Authors:
S Nakano; K Matsukado; K L Black
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurological research     Volume:  19     ISSN:  0161-6412     ISO Abbreviation:  Neurol. Res.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1997-11-19     Completed Date:  1997-11-19     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7905298     Medline TA:  Neurol Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  501-8     Citation Subset:  IM    
Affiliation:
Jonsson Comprehensive Cancer Center, Division of Neurosurgery, UCLA Medical Center 90095, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport / drug effects
Bradykinin / administration & dosage,  analogs & derivatives*,  pharmacology
Brain Neoplasms / metabolism*,  pathology
Capillary Permeability
Cytokines / pharmacokinetics*
Female
Glioma / metabolism*,  pathology
Infusions, Intra-Arterial
Intercellular Adhesion Molecule-1 / metabolism*
Interferon-gamma / pharmacokinetics
Interleukin-2 / pharmacokinetics
Iodine Radioisotopes / pharmacokinetics
Neoplasm Transplantation
Rats
Rats, Wistar
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha / pharmacokinetics
Grant Support
ID/Acronym/Agency:
1PO1NS25554/NS/NINDS NIH HHS; 1RO1NS32103/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Cytokines; 0/Interleukin-2; 0/Iodine Radioisotopes; 0/Tumor Necrosis Factor-alpha; 126547-89-5/Intercellular Adhesion Molecule-1; 159768-75-9/RMP 7; 58-82-2/Bradykinin; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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