Document Detail


Enhanced cytochrome-oxidase staining of the cuneate nucleus in the rat reveals a modifiable somatotopic map.
MedLine Citation:
PMID:  7687194     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Existing cytochrome oxidase (CO)-staining techniques were modified to enhance sensitivity and contrast in order to examine patterns of CO-activity in the dorsal column nuclei (DCN) of adult Long-Evans rats. Within a rostrocaudally limited region in the middle of the cuneate nucleus (CN) distinctive blotches of intense CO-activity were observed. The CO-staining was maximally differentiated approximately 0.3-0.7 mm caudal to the obex. No CO-blotches were observed anywhere else in the DCN. Transganglionic labelling (WGA-HRP) demonstrated that some of the CO-blotches in the rat CN are related to the terminal projection fields of primary afferents from the skin of the forepaws. The corresponding location of primary afferent termination fields and CO-staining patterns supports a tripartite rostrocaudal division in the rat CN, similar to that described by other investigators in cats, monkeys and raccoons. Comparing the patterns of CO-staining to (1) the cytoarchitecture (Nissl-stained sections), or to (2) the dendritoarchitecture (distribution of microtubule-associated protein 2 (MAP2) or to (3) the organization of retrogradely labelled (WGA-HRP/HRP) cuneothalamic cells, revealed no topographical organization corresponding to the CO-blotches. Postnatal (at least up to 11 days postpartum) forepaw deafferentation or removal disrupted the CO-staining pattern in the CN.
Authors:
D P Crockett; S Maslany; S L Harris; M D Egger
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Brain research     Volume:  612     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-08-13     Completed Date:  1993-08-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  41-55     Citation Subset:  IM    
Affiliation:
Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854-5635.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Brain Mapping*
Dendrites / ultrastructure
Electron Transport Complex IV / analysis*
Female
Ganglia / cytology,  enzymology
Histocytochemistry
Horseradish Peroxidase
Male
Medulla Oblongata / cytology,  enzymology,  physiology*
Nerve Fibers / enzymology
Neuronal Plasticity / physiology
Neurons, Afferent / physiology
Rats
Thalamus / cytology,  enzymology
Tissue Fixation
Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
Wheat Germ Agglutinins
Chemical
Reg. No./Substance:
0/Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; 0/Wheat Germ Agglutinins; EC 1.11.1.-/Horseradish Peroxidase; EC 1.9.3.1/Electron Transport Complex IV

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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