Document Detail


Enhanced core hydrophobicity, functionalization and cell penetration of polybasic nanomatrices.
MedLine Citation:
PMID:  18751960     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: In this work a novel pH-responsive nanoscale polymer network was investigated for potential applications in nanomedicine. These consisted of a polybasic core surface stabilized with poly(ethylene glycol) grafts. The ability to control swelling properties via changes in core hydrophobicity and crosslinking feed density was assessed. The nanomatrices were also evaluated in vitro as nanocarriers for targeted intracellular delivery of macromolecules.
MATERIALS AND METHODS: Photo-emulsion polymerization was used to synthesize poly[2-(diethylamino)ethyl methacrylate-co-t-butyl methacrylate-g-poly(ethylene glycol)] (PDBP) nanomatrices. These were characterized using NMR, dynamic and electrophoretic light scattering, electron microscopy. The cytocompatibility and cellular uptake of nanomatrices was measured using the NIH/3T3 and A549 cell lines.
RESULTS: PDBP nanomatrices had a dry diameter of 40-60 nm and a hydrodynamic diameter of 70-90 nm in the collapsed state. Maximum volume swelling ratios from 6-22 were obtained depending on crosslinking feed density. Controlling the hydrophobicity of the networks allowed for control over the critical swelling pH without a significant loss in maximal volume swelling. The effect of PDBP nanomatrices on cell viability and cell membrane integrity depended on crosslinking feed density. Cell uptake and cytosolic delivery of FITC-albumin was enhanced from clathrin-targeting nanocarriers. The uptake resulted in nuclear localization of the dye in a cell type dependent fashion.
CONCLUSIONS: The results of this work indicate that PDBP nanomatrices have tunable swelling properties. The networks were cytocompatible and proved to be suitable agents for intracellular delivery.
Authors:
Omar Z Fisher; Timothy Kim; Stephen R Dietz; Nicholas A Peppas
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-08-28
Journal Detail:
Title:  Pharmaceutical research     Volume:  26     ISSN:  0724-8741     ISO Abbreviation:  Pharm. Res.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-17     Completed Date:  2009-03-27     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  8406521     Medline TA:  Pharm Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  51-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Amines / chemistry
Animals
Biocompatible Materials
Cell Membrane / metabolism*
Cell Survival / drug effects
Fluorescein-5-isothiocyanate
Fluorescent Dyes
Hydrogen-Ion Concentration
Indicators and Reagents
Light
Magnetic Resonance Spectroscopy
Methacrylates / chemistry
Mice
Microscopy, Electron, Scanning
Nanoparticles / chemistry*
Nylons / chemistry
Particle Size
Polyethylene Glycols / chemistry*
Scattering, Radiation
Solubility
Surface Properties
Water
Grant Support
ID/Acronym/Agency:
EG-000246//PHS HHS; R01 EB000246/EB/NIBIB NIH HHS; R01 EB000246-15/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Amines; 0/Biocompatible Materials; 0/Fluorescent Dyes; 0/Indicators and Reagents; 0/Methacrylates; 0/Nylons; 0/Polyethylene Glycols; 0/poly(2-(diethylamino)ethyl methacrylate); 059QF0KO0R/Water; I223NX31W9/Fluorescein-5-isothiocyanate
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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