Document Detail


Enhanced collagen production by smooth muscle cells during repetitive mechanical stretching.
MedLine Citation:
PMID:  3178470     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined the effect of repetitive mechanical stretching on smooth muscle cell (SMC) collagen production. Porcine SMCs from passages 3 through 7 were seeded in 35-mm2 flexible-bottomed culture wells at a concentration of 2 x 10(5) cells per well and allowed to attach for 24 hours. The experimental group was placed in a vacuum-operated stress-providing instrument that exerted an average elongation of 25% at maximum downward deflection of the culture plate bottom. The stretched cells (nine wells per day) were subjected to a cyclic force regimen of 10 s of elongation and 10 s of relaxation for five days. The control cells (nine wells per day) were subjected to incubation conditions similar to those in the experimental group but without cyclic stretching. Twenty-four hours before harvesting, serum-free medium containing 50-microCi tritiated proline, an amino acid hydroxylated in collagen (hydroxyproline), and 50 micrograms/mL of ascorbate was added per well. On days 3 and 5 the medium and cells were collected, precipitated with trichloroacetic acid, and then sedimented, lyophilized, and analyzed to separate hydroxyproline and proline. Values for collagen and noncollagen protein were calculated after quantitation of the hydroxyproline and proline concentrations. The results indicate that three-cycle-per-minute stretching coordinately stimulated SMC production of collagen and noncollagen protein. We conclude that pulsatile stretch enhances collagen and noncollagen protein synthesis.
Authors:
B E Sumpio; A J Banes; W G Link; G Johnson
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Archives of surgery (Chicago, Ill. : 1960)     Volume:  123     ISSN:  0004-0010     ISO Abbreviation:  Arch Surg     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1988-11-07     Completed Date:  1988-11-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9716528     Medline TA:  Arch Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1233-6     Citation Subset:  AIM; IM; S    
Affiliation:
Department of Surgery, Yale University School of Medicine, New Haven, CT 06510.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aorta
Cells, Cultured
Collagen / biosynthesis*
Hydroxyproline / biosynthesis
Muscle, Smooth, Vascular / cytology*
Proline / metabolism
Protein Biosynthesis
Stress, Mechanical
Swine
Vacuum
Grant Support
ID/Acronym/Agency:
AM 30478/AM/NIADDK NIH HHS; AM 30952/AM/NIADDK NIH HHS; DE 02668/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
147-85-3/Proline; 51-35-4/Hydroxyproline; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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