Document Detail


Enhanced on-chip SERS based biomolecular detection using electrokinetically active microwells.
MedLine Citation:
PMID:  19156293     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Here we present a novel microfluidic technique for on-chip surface enhanced Raman spectroscopy (SERS) based biomolecular detection, exploiting the use of electrokinetically active microwells. Briefly, the chip comprises of a series of microfluidic channels containing embedded microwells that, when electrically actuated, either locally attract or repulse species from solution through a combination of electrokinetic effects. We demonstrate that the approach combines the advantages of existing homogeneous (solution phase) and heterogeneous (surface phase) on-chip techniques by enabling active mixing to enhance the rate of binding between the SERS enhancers and the biomolecular targets as well as rapid concentration of the product for surface phase optical interrogation. This paper describes the chip design and fabrication procedure, experimental results illustrating the optimal conditions for our concentration and mixing processes, and a numerical analysis of the flow pattern. To demonstrate the usefulness of the device we apply it to the quantitative detection of nucleic acid sequences associated with Dengue virus serotype 2. We report a limit of detection for Dengue sequences of 30 pM and show excellent specificity against other serotypes.
Authors:
Yun Suk Huh; Aram J Chung; Bernardo Cordovez; David Erickson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-11-12
Journal Detail:
Title:  Lab on a chip     Volume:  9     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-21     Completed Date:  2009-05-14     Revised Date:  2013-11-12    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  433-9     Citation Subset:  IM    
Affiliation:
Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY 14853, USA. de54@cornell.edu.
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MeSH Terms
Descriptor/Qualifier:
DNA, Viral / analysis
Dengue Virus / chemistry
Electrochemical Techniques / methods*
Gold / chemistry
Microfluidic Analytical Techniques / methods*
Nanoparticles / chemistry
Polystyrenes / chemistry
Sensitivity and Specificity
Spectrum Analysis, Raman / methods*
Surface Properties
Grant Support
ID/Acronym/Agency:
R21 EB007031/EB/NIBIB NIH HHS; R21 EB007031-02/EB/NIBIB NIH HHS; R21EB007031/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/Polystyrenes; 7440-57-5/Gold
Comments/Corrections

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