Document Detail

Enhanced capacity of n-3 fatty acid-enriched macrophages to oxidize low density lipoprotein mechanisms and effects of antioxidant vitamins.
MedLine Citation:
PMID:  8830929     Owner:  NLM     Status:  MEDLINE    
We have investigated possible mechanisms by which n-3 fatty acid-enriched macrophages enhance the oxidation of low density lipoprotein (LDL), and the ability of antioxidant vitamins to prevent this. Macrophages were enriched with n-3 fatty acids (eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid) following incubation with fish oil. These macrophages produced large amount of TBARS in medium containing metals, and showed enhanced capacity to oxidize LDL (3-4 fold increase compared to control cells) and to accumulate the modified LDL. 5,8,11,14-eicosatetraynoic acid (ETYA, 15-lipoxygenase inhibitor) and superoxide dismutase (SOD) did not inhibit the enhanced capacity of n-3 fatty acid-enriched cells to oxidize LDL. However antioxidants, (vitamin E-enriched macrophages or vitamin C in the medium), inhibited this enhanced capacity. Medium conditioned by n-3 fatty acid-enriched cells had pro-oxidant effects on metal-initiated LDL oxidation. We conclude that n-3 fatty acid-enriched macrophages display increased oxidant capacity which is not inhibited by ETYA or SOD, and that antioxidant vitamins inhibit the enhanced capacity to oxidize LDL.
M Suzukawa; M Abbey; P Clifton; P J Nestel
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Atherosclerosis     Volume:  124     ISSN:  0021-9150     ISO Abbreviation:  Atherosclerosis     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1996-12-11     Completed Date:  1996-12-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  157-69     Citation Subset:  IM    
CSIRO, Division of Human Nutrition, Adelaide, Australia.
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MeSH Terms
5,8,11,14-Eicosatetraynoic Acid / pharmacology
Antioxidants / pharmacology*
Arteriosclerosis / metabolism,  pathology,  prevention & control
Cell Culture Techniques
Chromatography, Gas
Fatty Acids, Omega-3 / pharmacology*
Lipid Peroxidation / drug effects,  physiology*
Lipoproteins, LDL / drug effects,  metabolism*
Macrophages / drug effects,  metabolism*,  pathology
Superoxide Dismutase / pharmacology
Thiobarbituric Acid Reactive Substances / metabolism
Reg. No./Substance:
0/Antioxidants; 0/Fatty Acids, Omega-3; 0/Lipoproteins, LDL; 0/Thiobarbituric Acid Reactive Substances; 1191-85-1/5,8,11,14-Eicosatetraynoic Acid; EC Dismutase

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