Document Detail


Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid.
MedLine Citation:
PMID:  16430509     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: The aim of the present study was to isolate and characterize a bacterium, strain EN3, capable of using diesel oil as a major carbon and energy source, and to analyse the enhancement of diesel oil degradation by this organism using synthetic mycolic acid (2-hexyl-3-hydroxyldecanoic acid). METHOD AND RESULTS: An actinomycete with the ability to degrade diesel oil was isolated from oil contaminated soil and characterized. The strain had phenotypic properties consistent with its classification in the genus Rhodococcus showing a 16S rRNA gene similarity of 99.7% with Rhodococcus baikonurensis DSM 44587(T). The ability of the characterized strain to degrade diesel oil at various concentrations (1000, 5000, 10 000 and 20 000 mg l(-1)) was determined. The effect of synthetic mycolic acid on the biodegradation of diesel oil was investigated at the 20 000 mg l(-1) concentration; the surfactant was added to the flask cultures at three different concentrations (10, 50 and 100 mg l(-1)) and degradation followed over 7 days. Enhanced degradation was found at all three concentrations of the surfactant. In addition, the enhancement of diesel oil degradation by other surfactants was observed. CONCLUSIONS: The synthetic mycolic acid has potential for the remediation of petroleum-contaminated sites from both an economic and applied perspective as it can stimulate biodegradation at low concentrations. SIGNIFICANCE AND IMPACT OF THE STUDY: This study showed that the synthesized mycolic acid can be used for potential applications in the bioremediation industries, for example, in oil spill clean-up, diesel fuel remediation and biostimulation.
Authors:
M Lee; M K Kim; I Singleton; M Goodfellow; S-T Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied microbiology     Volume:  100     ISSN:  1364-5072     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-24     Completed Date:  2006-09-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  325-33     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea. e_stlee@kaist.ac.kr
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MeSH Terms
Descriptor/Qualifier:
Biodegradation, Environmental / drug effects
Culture Media
Gasoline*
Genes, Bacterial / genetics
Mycolic Acids / pharmacology*
Phenotype
Phylogeny
RNA, Ribosomal, 16S / genetics
Rhodococcus / genetics,  metabolism*
Soil Microbiology
Soil Pollutants / metabolism*
Surface-Active Agents / pharmacology*
Chemical
Reg. No./Substance:
0/2-hexyl-3-hydroxydecanoic acid; 0/Culture Media; 0/Gasoline; 0/Mycolic Acids; 0/RNA, Ribosomal, 16S; 0/Soil Pollutants; 0/Surface-Active Agents

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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