Document Detail

Enhanced apoptosis in transformed human lung fibroblasts after exposure to sodium butyrate.
MedLine Citation:
PMID:  8889605     Owner:  NLM     Status:  MEDLINE    
Simian virus-transformed human cells, WI-38 VA13A, showed a dose-dependent induction of apoptosis and reduction in cell numbers after exposure to sodium butyrate. Apoptosis was confirmed by ApopTag staining, isolation of apoptotic envelopes, and immunofluorescent staining with an antibody specific for apoptotic envelopes. Examination of the cell cultures by phase contrast and fluorescent microscopy revealed the presence of enlarged cells that displayed a more flattened morphology and morphological changes in the nucleus of cells exposed to sodium butyrate. Cell proliferation assays showed control and sodium butyrate cultures were synthesizing DNA and excluded any cytotoxic effects of sodium butyrate. Flow cytometry results indicated an increase in the number of aneuploid cells following sodium butyrate treatment. There was a decrease in the percentage of cells in G2/M in the diploid populations, but an increase in the percentage of cells in G2/ M in aneuploid populations. This human in vitro model system suggests a mode of action for the therapeutic effects of sodium butyrate, which have been observed in the topical treatment of neoplastic cells and reversal of symptom in ulcerative colitis, namely, the induction of apoptosis.
G L Thomas; A Henley; T C Rowland; A Sahai; M Griffin; P J Birckbichler
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  32     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1997-02-03     Completed Date:  1997-02-03     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  505-13     Citation Subset:  IM    
Oklahoma Medical Research Foundation, Noble Center for Biomedical Research, Oklahoma City 73104, USA.
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MeSH Terms
Biological Markers / analysis
Butyric Acid
Butyric Acids / toxicity*
Cell Cycle / drug effects*
Cell Division / drug effects
Cell Line, Transformed
Cell Nucleus / drug effects,  ultrastructure
Fibroblasts / drug effects
Fluorescent Antibody Technique, Indirect
Transglutaminases / analysis
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Biological Markers; 0/Butyric Acids; 107-92-6/Butyric Acid; EC

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