Document Detail


Enhanced anabolic response to milk protein sip feeding in elderly subjects with COPD is associated with a reduced splanchnic extraction of multiple amino acids.
MedLine Citation:
PMID:  22682082     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND & AIMS: We previously observed in elderly subjects with Chronic Obstructive Pulmonary Disease (COPD) an enhanced anabolic response to milk protein sip feeding, associated with reduced splanchnic extraction (SPE) of phenylalanine. Milk proteins are known for their high Branched-chain Amino Acids (BCAA) content, but no information is present about splanchnic extraction and metabolism of the individual BCAA in COPD.
OBJECTIVE: To investigate whether BCAA metabolism and SPE of the individual BCAA are altered in COPD during milk protein sip feeding.
DESIGN: In elderly subjects with COPD and in healthy age-matched elderly SPE, endogenous rate of appearance (Raendo) of the leucine (LEU), isoleucine (ILE) and valine (VAL) were measured before and during sip feeding of a Whey protein meal. To study the effect of aging, the healthy elderly were compared to a group of healthy young subjects. Stable isotopes of l-[(2)H(3)]-LEU, l-[1-(13)C]-ILE and l-[1-(13)C]-VAL were given on two separate test days orally or intravenously. Simultaneously, l-[ring-(2)H(5)]-phenylalanine (PHE) and l-[ring-(2)H(2)]-tyrosine (TYR) were given to determine the whole body protein breakdown (WbPB), synthesis (WbPS) and NetPS.
RESULTS: SPE of all BCAA, TYR, and PHE (p < 0.01) were lower in the COPD group, and the increase in netPS during feeding was higher in the COPD group (P < 0.01) due to higher values for PS (P < 0.001). Raendo of all BCAA, PHE and TYR were higher in the COPD than the healthy elderly group (P < 0.05) before and during feeding (P < 0.001). Sip feeding resulted in a reduction of Raendo of PHE, ILE and VAL (P < 0.05). Postabsorptive Raendo was not different for any of the measured amino acids between the healthy elderly and young group, while sip feeding resulted in a reduction of Raendo of PHE. Only SPE of TYR was higher in the elderly (P < 0.05) and the increase in netPS during sip feeding was independent of aging.
CONCLUSION: The enhanced anabolic response to milk protein sip feeding in normal-weight COPD patients is associated with a reduced splanchnic extraction of multiple amino acids including all branched-chain amino acids. Registration ClinicalTrials.gov = NCT01418469.
Authors:
M P K J Engelen; C L N De Castro; E P A Rutten; E F M Wouters; A M W J Schols; N E P Deutz
Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-06-06
Journal Detail:
Title:  Clinical nutrition (Edinburgh, Scotland)     Volume:  31     ISSN:  1532-1983     ISO Abbreviation:  Clin Nutr     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-28     Completed Date:  2013-03-25     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  8309603     Medline TA:  Clin Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  616-24     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
Affiliation:
Center for Translational Research in Aging & Longevity, Donald W. Reynolds Institute on Aging, University of Arkansas for Medical Sciences, Little Rock, AR, USA. mpkj.engelen@ctral.org
Data Bank Information
Bank Name/Acc. No.:
ClinicalTrials.gov/NCT01418469
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MeSH Terms
Descriptor/Qualifier:
Administration, Intravenous
Administration, Oral
Aged
Amino Acids, Branched-Chain / administration & dosage*,  blood
Body Composition
Body Weight
Humans
Isoleucine / administration & dosage,  analysis
Leucine / administration & dosage,  analysis
Male
Meals
Middle Aged
Milk Proteins / administration & dosage*,  chemistry
Phenylalanine / administration & dosage,  analysis
Pulmonary Disease, Chronic Obstructive / drug therapy*,  physiopathology*
Tyrosine / administration & dosage,  analysis
Valine / administration & dosage,  analysis
Young Adult
Grant Support
ID/Acronym/Agency:
R-01HL095903/HL/NHLBI NIH HHS; R01 HL095903/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids, Branched-Chain; 0/Milk Proteins; 0/whey protein; 55520-40-6/Tyrosine; 61-90-5/Leucine; 63-91-2/Phenylalanine; 7004-03-7/Valine; 73-32-5/Isoleucine
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