Document Detail


Enhanced airway responses to substance P after repeated challenge in guinea pigs.
MedLine Citation:
PMID:  2468638     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We performed three consecutive dose-response curves to rapid intravenous infusions of substance P (SP) in anesthetized, mechanically ventilated guinea pigs. The dose of SP required to decrease pulmonary conductance to 50% of its base-line value (ED50GL) decreased 2.8-fold (P less than 0.002) and 3.3-fold (P less than 0.001) on the second and third dose-response curves, respectively, compared with the first. SP did not alter airway responses to intravenous histamine but did cause a significant (3.7-fold) decrease in ED50GL for dose-response curves to intravenous capsaicin, an agent that causes bronchoconstriction by release of endogenous tachykinins. The neutral metalloendopeptidase inhibitor thiorphan (0.5 mg) and the angiotensin-converting enzyme inhibitor captopril (1.7 mg) both caused a marked enhancement of airway responses to SP observed on the first dose-response curve but did not alter the enhancement of SP-induced airway responses produced by repeated SP challenge. The anticholinergic atropine (5 mg/kg iv), the antihistamine mepyramine (8 mg/kg iv), and the cyclooxygenase inhibitor indomethacin (30 mg/kg ip) had no effect on the first SP dose-response curve. Atropine and mepyramine did not prevent the enhancement of SP responses observed with repeated challenge, but after pretreatment with either indomethacin or acetylsalicylic acid, dose-response curves to SP were reproducible. Our results indicate that airway responses to intravenous SP are enhanced with repeated SP challenge and suggest that cyclooxygenase products of arachidonic acid metabolism are involved in the mediation of this phenomenon.
Authors:
S A Shore; J M Drazen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  66     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-06-02     Completed Date:  1989-06-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  955-61     Citation Subset:  IM    
Affiliation:
Department of Medicine, Beth Israel Hospital, Boston, Massachusetts.
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MeSH Terms
Descriptor/Qualifier:
Animals
Atropine / pharmacology
Capsaicin / pharmacology
Dose-Response Relationship, Drug
Guinea Pigs
Histamine / pharmacology
Indomethacin / pharmacology
Lung Compliance / drug effects
Male
Muscle Contraction / drug effects
Pyrilamine / pharmacology
Respiratory Physiological Phenomena
Respiratory System / drug effects*
Substance P / administration & dosage,  pharmacology*
Grant Support
ID/Acronym/Agency:
HL-39827/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
33507-63-0/Substance P; 404-86-4/Capsaicin; 51-45-6/Histamine; 51-55-8/Atropine; 53-86-1/Indomethacin; 91-84-9/Pyrilamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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