Document Detail

Enhanced activity of very low density lipoprotein receptor II promotes SGC7901 cell proliferation and migration.
MedLine Citation:
PMID:  19167408     Owner:  NLM     Status:  MEDLINE    
AIMS: Clear differences of biological function between very low density lipoprotein receptor (VLDLR) types I and II have not been defined. The purpose of this study is to characterize VLDLR activities during cell proliferation and migration using adenocarcinoma SGC7901 cells. MAIN METHODS: Western blotting was used to test protein expression. Cell proliferation or migration was analyzed by MTT or Transwell chambers respectively. The mRNA expression was tested by RT-PCR. Reporter assay was to test the transcription activity. KEY FINDINGS: The cells treated with all-trans retinoic acid (ATRA) became well differentiated and had a gradually attenuated cell proliferation and migration, accompanied by a significant decrease in type II VLDLR expression. These cells also had decreased amount of beta-catenin, indicating a decreased stability of beta-catenin. In addition, the mRNA expression of both matrix metalloproteinase (MMP)-2 and MMP-9 was also decreased. On the contrary, cells treated with phorbol-12-myristate-13-acetate (PMA) had an increase in type II VLDLR expression, whereas the beta-catenin was increased. This was accompanied by increased cell proliferation and migration and by increased MMP-2 and MMP-9 mRNA expression. Finally, the transfection of SGC7901 cells with type II VLDLR recombinant DNA induced the cell proliferation and migration as well as the activation of beta-catenin/T cell factor (TCF) signaling. However, type I VLDLR transfection reduced beta-catenin stability and decreased cell proliferation and migration. SIGNIFICANCE: These data strongly suggest that type II VLDLR activity is positively associated with significant change of tumor cell proliferation and migration, via the beta-catenin/TCF signaling.
Pu Yang; Zhiguo Liu; Hongxing Wang; Jun Tian; Yinghong Li; Yiqiang Zong; Shen Qu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-08
Journal Detail:
Title:  Life sciences     Volume:  84     ISSN:  1879-0631     ISO Abbreviation:  Life Sci.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-17     Completed Date:  2009-05-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  402-8     Citation Subset:  IM    
Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
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MeSH Terms
Blotting, Western
Cell Line, Tumor
Cell Movement / drug effects,  physiology*
Cell Proliferation* / drug effects
Matrix Metalloproteinase 2 / metabolism
Matrix Metalloproteinase 9 / metabolism
RNA, Messenger / biosynthesis
Receptors, LDL / biosynthesis,  metabolism*,  physiology
Reverse Transcriptase Polymerase Chain Reaction
Tetradecanoylphorbol Acetate / pharmacology
Tretinoin / pharmacology
beta Catenin / metabolism
Reg. No./Substance:
0/Ligands; 0/RNA, Messenger; 0/Receptors, LDL; 0/beta Catenin; 0/very low density lipoprotein receptor type II, human; 16561-29-8/Tetradecanoylphorbol Acetate; 302-79-4/Tretinoin; EC Metalloproteinase 2; EC Metalloproteinase 9

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