| Enhanced activity of very low density lipoprotein receptor II promotes SGC7901 cell proliferation and migration. | |
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MedLine Citation:
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PMID: 19167408 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: Clear differences of biological function between very low density lipoprotein receptor (VLDLR) types I and II have not been defined. The purpose of this study is to characterize VLDLR activities during cell proliferation and migration using adenocarcinoma SGC7901 cells. MAIN METHODS: Western blotting was used to test protein expression. Cell proliferation or migration was analyzed by MTT or Transwell chambers respectively. The mRNA expression was tested by RT-PCR. Reporter assay was to test the transcription activity. KEY FINDINGS: The cells treated with all-trans retinoic acid (ATRA) became well differentiated and had a gradually attenuated cell proliferation and migration, accompanied by a significant decrease in type II VLDLR expression. These cells also had decreased amount of beta-catenin, indicating a decreased stability of beta-catenin. In addition, the mRNA expression of both matrix metalloproteinase (MMP)-2 and MMP-9 was also decreased. On the contrary, cells treated with phorbol-12-myristate-13-acetate (PMA) had an increase in type II VLDLR expression, whereas the beta-catenin was increased. This was accompanied by increased cell proliferation and migration and by increased MMP-2 and MMP-9 mRNA expression. Finally, the transfection of SGC7901 cells with type II VLDLR recombinant DNA induced the cell proliferation and migration as well as the activation of beta-catenin/T cell factor (TCF) signaling. However, type I VLDLR transfection reduced beta-catenin stability and decreased cell proliferation and migration. SIGNIFICANCE: These data strongly suggest that type II VLDLR activity is positively associated with significant change of tumor cell proliferation and migration, via the beta-catenin/TCF signaling. |
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Authors:
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Pu Yang; Zhiguo Liu; Hongxing Wang; Jun Tian; Yinghong Li; Yiqiang Zong; Shen Qu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-01-08 |
Journal Detail:
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Title: Life sciences Volume: 84 ISSN: 1879-0631 ISO Abbreviation: Life Sci. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-03-17 Completed Date: 2009-05-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: England |
Other Details:
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Languages: eng Pagination: 402-8 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western Cell Line, Tumor Cell Movement / drug effects, physiology* Cell Proliferation* / drug effects Humans Ligands Matrix Metalloproteinase 2 / metabolism Matrix Metalloproteinase 9 / metabolism RNA, Messenger / biosynthesis Receptors, LDL / biosynthesis, metabolism*, physiology Reverse Transcriptase Polymerase Chain Reaction Tetradecanoylphorbol Acetate / pharmacology Tretinoin / pharmacology beta Catenin / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Ligands; 0/RNA, Messenger; 0/Receptors, LDL; 0/beta Catenin; 0/very low density lipoprotein receptor type II, human; 16561-29-8/Tetradecanoylphorbol Acetate; 302-79-4/Tretinoin; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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