| Enhanced acetoin production by Serratia marcescens H32 with expression of a water-forming NADH oxidase. | |
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MedLine Citation:
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PMID: 22728188 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Cofactor engineering was employed to enhance production of acetoin by Serratia marcescens H32. 2,3-Butanediol was a major byproduct of acetoin fermentation by S. marcescens H32. In order to decrease 2,3-butanediol formation and achieve a high efficiency of acetoin production, nox gene encoding a water-forming NADH oxidase from Lactobacillus brevis was expressed. Batch fermentations suggested the expression of the NADH oxidase could increase the intracellular NAD(+) concentration (1.5-fold) and NAD(+)/NADH ratio (2.9-fold). Meanwhile, 2,3-butanediol was significantly decreased (52%), and the accumulation of acetoin was enhanced (33%) accordingly. By fed-batch culture of the engineered strain, the final acetoin titer up to 75.2g/l with the productivity of 1.88g/(lh) was obtained. To the best of our knowledge, these results were new records on acetoin fermentation ever reported. |
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Authors:
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Jian-An Sun; Liao-Yuan Zhang; Ben Rao; Ya-Ling Shen; Dong-Zhi Wei |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-30 |
Journal Detail:
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Title: Bioresource technology Volume: 119C ISSN: 1873-2976 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-6-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9889523 Medline TA: Bioresour Technol Country: - |
Other Details:
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Languages: ENG Pagination: 94-98 Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Meilong Road 130, Shanghai 200237, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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