Document Detail


Enhanced FGF-2 movement through human sclera after exposure to latanoprost.
MedLine Citation:
PMID:  11581197     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To determine whether exposure of sclera to latanoprost acid alters transscleral permeation by FGF-2. METHODS: Pieces of human sclera were isolated from donor eyes after death, placed in organ culture, and exposed to 50 to 200 nM latanoprost acid or vehicle for 3 days. Transscleral permeability was then assessed by placing each scleral piece into a Ussing apparatus and measuring the amount of FGF-2 that moves from the orbital side to the uveal side of the scleral piece. Transscleral permeation by 10-kDa tetramethylrhodamine-dextran also was determined, for comparison. RESULTS: Transscleral permeation by FGF-2 through sclera that had been incubated with vehicle was 1.53 +/- 0.86 x 10(-8) cm/sec. Transscleral permeation by 10-kDa tetramethylrhodamine-dextran was 1.04 +/- 0.39 x 10(-6) cm/sec. FGF-2 permeation of sclera exposed to 50, 100, and 200 nM latanoprost acid was increased by an average of 48% +/- 62%, 100% +/- 108%, and 108% +/- 79%, respectively, compared with sclera exposed to vehicle (n = 13; P < 0.05). Scleral permeation by 10-kDa dextran after exposure to 50, 100, or 200 nM latanoprost acid was significantly increased by 42% +/- 36%, 59% +/- 51%, and 65% +/- 49%, respectively (n = 14; P < 0.05). The ratio of dextran to FGF-2 permeation was approximately 90 and did not vary with 50, 100, or 200 nM latanoprost acid (P = 0.93, ANOVA). CONCLUSIONS: Exposure of sclera to latanoprost acid increases transscleral permeation by FGF-2 in human scleral organ cultures. Because this increase parallels the increased scleral permeability caused by dextran, it may reflect a general enhancement of permeability, a possibility that future in vivo studies should explore.
Authors:
M Aihara; J D Lindsey; R N Weinreb
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  42     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-10-02     Completed Date:  2001-10-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2554-9     Citation Subset:  IM    
Affiliation:
Glaucoma Center, University of California San Diego, La Jolla 92093-0946, USA.
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MeSH Terms
Descriptor/Qualifier:
Aged
Antihypertensive Agents / pharmacology*
Dextrans / pharmacology
Dose-Response Relationship, Drug
Female
Fibroblast Growth Factor 2 / metabolism*
Humans
Male
Middle Aged
Organ Culture Techniques
Permeability
Prostaglandins F, Synthetic / pharmacology*
Rhodamines / pharmacology
Sclera / drug effects*,  metabolism
Time Factors
Grant Support
ID/Acronym/Agency:
EY05990/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Antihypertensive Agents; 0/Fluoro-Ruby; 0/Prostaglandins F, Synthetic; 0/Rhodamines; 103107-01-3/Fibroblast Growth Factor 2; 130209-82-4/latanoprost; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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