Document Detail


Enhanced electron transfer activity of photosystem I by polycations in aqueous solution.
MedLine Citation:
PMID:  20882997     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The use of proteins in advanced nanotechnological applications requires extended stabilization of the functional protein conformation and enhanced activity. Here we report that simple cationic poly(amino acid)s can significantly increase the activity of the multidomain protein supercomplex Photosystem-I (PS-I) in solution better than other commonly used chemical detergents and anionic poly(amino acid)s. We carried out a systematic analysis using a series of poly(amino acid)s (i.e., poly-l-tyrosine, poly-l-histidine, poly-l-aspartic and poly-l-glutamic acid, poly-l-arginine, and poly-l-lysine). Our results show that the polycations poly-l-lysine and poly-l-arginine significantly enhance the photochemical activity of PS-I, whereas negatively charged and hydrophobic poly(amino acid)s did not increase the PS-I functionality in solution. Furthermore, we show that poly-l-lysine can stabilize highly active PS-I in the dry state, resulting in 84% activity recovery. These simple and inexpensive poly(amino acid)s will likely make significant contributions toward a highly active form of the PS-I membrane protein with important applications in nanotechnology and biotechnology.
Authors:
Kazuya Matsumoto; Shuguang Zhang; Sotirios Koutsopoulos
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Publication Detail:
Type:  Journal Article     Date:  2010-09-30
Journal Detail:
Title:  Biomacromolecules     Volume:  11     ISSN:  1525-7797     ISO Abbreviation:  Biomacromolecules     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2014-02-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3152-7     Citation Subset:  IM    
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