Document Detail


Enhanced DNA transfer into fish bone cells using polyethylenimine.
MedLine Citation:
PMID:  16943571     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of in vitro cell culture systems to assess gene function largely depends on the successful transfer of DNA into target cells. Well developed in mammals, transfection methods are still to be optimized for non-mammalian cell culture systems, like fish. Here we describe a rapid, cost-efficient, and successful method to transfer DNA into a fish bone-derived cell line using polyethylenimine (PEI) as the DNA carrier. Using this method, DNA transfer was remarkably enhanced in comparison with commercially available reagents, as demonstrated by the increased activity of both luciferase and green fluorescent protein observed in the transfected cells. Its efficiency in transferring DNA into a wide range of cell types, including non-mammalian and hard-to-transfect cells, in addition to a low cost, show that PEI is a reagent of choice for nonviral vector transfection.
Authors:
Daniel Braga; Vincent Laizé; Daniel M Tiago; M Leonor Cancela
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular biotechnology     Volume:  34     ISSN:  1073-6085     ISO Abbreviation:  Mol. Biotechnol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-31     Completed Date:  2006-10-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  51-4     Citation Subset:  IM    
Affiliation:
Centre of Marine Sciences (CCMAR), University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques / methods
Cells, Cultured
Coated Materials, Biocompatible / chemistry
DNA / administration & dosage*,  genetics*
Drug Carriers / chemistry
Materials Testing
Osteoblasts / metabolism*
Polyethyleneimine / chemistry*
Sea Bream / genetics*,  metabolism*
Transfection / methods*
Chemical
Reg. No./Substance:
0/Coated Materials, Biocompatible; 0/Drug Carriers; 9002-98-6/Polyethyleneimine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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